Figure 5. Decrease in protein concentration and heme content.

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Protein concentration
was determined using the Bradford method. Specific heme content
was set as absorbance at l=403 (see Figure 3) divided by protein concentration
and is displayed in arbitrary units. Protein concentration was determined
in triplicate.

HRP bradford and specific heme content plasma 20180214.xlsx

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Fig. 2 Kinetics of H2O2 accumulation and substrate conversion by HRP during direct plasma treatment.
Figure 3. HRP inactivation by plasma treatment
Figure 4. CD spectra of HRP after exposure to plasma
Figure 5. Decrease in protein concentration and heme content.
Figure 6. Influence of voltage and frequency on HRP after 1 min plasma treatment
Figure 7. Immobilization of HRP protects against plasma-mediated damage
Figure 8. Production of (R)-1-phenylethanol with immobilized rAaeUPO using plasma-treated KPi buffer (250 mm) treated for several cycles
Supplementary Figure 1. Background activity of direct biocatalysis using HRP.
Supplementary Figure 2. Comparison of active and inactive HRP in direct catalysis.
Supplementary Figure 3. Spectrum of reaction products of HRP and guaiacol.
Supplementary Figure 4. Conversion of substrates by plasma in the absence of enzyme.
Supplementary Figure 5. HRP inactivation kinetics at different protein concentrations during treatment.
Supplementary Figure 6. Influence of SodA on HRP activity.
Supplementary Figure 7. Relative activity of HRP after plasma treatment with and without mannitol.
Supplementary Figure 8. Relative activity of free and immobilized HRP.
Supplementary Figure 9. Analysis of reaction products of rAaeUPO and plasma-treated buffer.
Supplementary Figure 10. Relative activity of rAaeUPO after incubation in plasmatreated buffer.
Supplementary Figure 11. Relative activity of rAaeUPO after plasma treatment.
Supplementary Figure 12. Relative activity of rAaeUPO after plasma treatment in the presence of SodA from E. coli.
Supplementary Figure 13. Inactivation of immobilized rAaeUPO.
Supplementary Figure 14. Relative activity of rAaeUPO before and after immobilization.
Supplementary Figure 15. Conversion of ethylbenzene to (R)-1-phenylethanol with immobilized rAaeUPO.

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timestamp	Nov 19, 2020