Primary tabs

Supplementary Figure 2. Comparison of active and inactive HRP in direct catalysis.

order to extract the heme from HRP, acidified enzyme solution was incubated with an excess
of ethyl acetate for 1 min. After centrifugation, the aqueous phase was transferred and protein
content was determined by the Bradford method. Catalysis was then carried out using
untreated (control) and heme-depleted HRP in 5 mM of guaiacol in 50 mM KPi buffer. After 5
min of plasma treatment, absorption at 470 nm was determined and normalized to protein
content of the sample as well as treatment time. The experiment was performed 3 times

Data preview unavailable.
Resource Quantity: 


Additional Information

filesize44.17 KB
resource typefile upload
timestampNov 18, 2020