order to extract the heme from HRP, acidified enzyme solution was incubated with an excess
of ethyl acetate for 1 min. After centrifugation, the aqueous phase was transferred and protein
content was determined by the Bradford method. Catalysis was then carried out using
untreated (control) and heme-depleted HRP in 5 mM of guaiacol in 50 mM KPi buffer. After 5
min of plasma treatment, absorption at 470 nm was determined and normalized to protein
content of the sample as well as treatment time. The experiment was performed 3 times
Supplementary Figure 2. Comparison of active and inactive HRP in direct catalysis.
- Fig. 2 Kinetics of H2O2 accumulation and substrate conversion by HRP during direct plasma treatment.
- Figure 3. HRP inactivation by plasma treatment
- Figure 4. CD spectra of HRP after exposure to plasma
- Figure 5. Decrease in protein concentration and heme content.
- Figure 6. Influence of voltage and frequency on HRP after 1 min plasma treatment
- Figure 7. Immobilization of HRP protects against plasma-mediated damage
- Figure 8. Production of (R)-1-phenylethanol with immobilized rAaeUPO using plasma-treated KPi buffer (250 mm) treated for several cycles
- Supplementary Figure 1. Background activity of direct biocatalysis using HRP.
- Supplementary Figure 2. Comparison of active and inactive HRP in direct catalysis.
- Supplementary Figure 3. Spectrum of reaction products of HRP and guaiacol.
- Supplementary Figure 4. Conversion of substrates by plasma in the absence of enzyme.
- Supplementary Figure 5. HRP inactivation kinetics at different protein concentrations during treatment.
- Supplementary Figure 6. Influence of SodA on HRP activity.
- Supplementary Figure 7. Relative activity of HRP after plasma treatment with and without mannitol.
- Supplementary Figure 8. Relative activity of free and immobilized HRP.
- Supplementary Figure 9. Analysis of reaction products of rAaeUPO and plasma-treated buffer.
- Supplementary Figure 10. Relative activity of rAaeUPO after incubation in plasmatreated buffer.
- Supplementary Figure 11. Relative activity of rAaeUPO after plasma treatment.
- Supplementary Figure 12. Relative activity of rAaeUPO after plasma treatment in the presence of SodA from E. coli.
- Supplementary Figure 13. Inactivation of immobilized rAaeUPO.
- Supplementary Figure 14. Relative activity of rAaeUPO before and after immobilization.
- Supplementary Figure 15. Conversion of ethylbenzene to (R)-1-phenylethanol with immobilized rAaeUPO.
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|timestamp||Nov 18, 2020|