Plasma treatment was performed
with 110 mL HRP (1 kUmL@1) in KPi buffer (100 mm, pH 6.5) without
guaiacol. Activity was measured by diluting the treated samples to
0.1 UmL@1 in KPi buffer including 5 mm of guaiacol and subsequent addition
of H2O2 at a final concentration of 0.25 mm. Guaiacol conversion was immediately
monitored at l=470 nm and activity calculated from the initial slope
during the first 30 s. From the same treated samples, absorption spectra
were measured with a 1:5 dilution in KPi. The inset shows the Soret band of
HRP with Amax at &403 nm. Spectra are displayed with the untreated sample
as blank, thereby showing a decrease in absorbance at the Soret peak. Data
were recorded in triplicate for both activity measurements and spectra.
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Figure 3. HRP inactivation by plasma treatment
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- Fig. 2 Kinetics of H2O2 accumulation and substrate conversion by HRP during direct plasma treatment.
- Figure 3. HRP inactivation by plasma treatment
- Figure 4. CD spectra of HRP after exposure to plasma
- Figure 5. Decrease in protein concentration and heme content.
- Figure 6. Influence of voltage and frequency on HRP after 1 min plasma treatment
- Figure 7. Immobilization of HRP protects against plasma-mediated damage
- Figure 8. Production of (R)-1-phenylethanol with immobilized rAaeUPO using plasma-treated KPi buffer (250 mm) treated for several cycles
- Supplementary Figure 1. Background activity of direct biocatalysis using HRP.
- Supplementary Figure 2. Comparison of active and inactive HRP in direct catalysis.
- Supplementary Figure 3. Spectrum of reaction products of HRP and guaiacol.
- Supplementary Figure 4. Conversion of substrates by plasma in the absence of enzyme.
- Supplementary Figure 5. HRP inactivation kinetics at different protein concentrations during treatment.
- Supplementary Figure 6. Influence of SodA on HRP activity.
- Supplementary Figure 7. Relative activity of HRP after plasma treatment with and without mannitol.
- Supplementary Figure 8. Relative activity of free and immobilized HRP.
- Supplementary Figure 9. Analysis of reaction products of rAaeUPO and plasma-treated buffer.
- Supplementary Figure 10. Relative activity of rAaeUPO after incubation in plasmatreated buffer.
- Supplementary Figure 11. Relative activity of rAaeUPO after plasma treatment.
- Supplementary Figure 12. Relative activity of rAaeUPO after plasma treatment in the presence of SodA from E. coli.
- Supplementary Figure 13. Inactivation of immobilized rAaeUPO.
- Supplementary Figure 14. Relative activity of rAaeUPO before and after immobilization.
- Supplementary Figure 15. Conversion of ethylbenzene to (R)-1-phenylethanol with immobilized rAaeUPO.
Additional Information
| Field | Value |
|---|---|
| mimetype | application/vnd.openxmlformats-officedocument.spreadsheetml.sheet |
| filesize | 427.93 KB |
| resource type | file upload |
| timestamp | Nov 19, 2020 |