100 μl of 100 mM pyrogallol, 10 mM guaiacol, and 20 mM L-DOPA in 100 mM KPi buffer were
treated with plasma for the indicated amounts of time. After treatment, absorption was
A mixture
of 0.1 U ml-1 HRP and 5 mM of guaiacol in 50 mM KPi buffer was either treated with the DBD
Reactions of 100 μl were run without enzyme, without plasma treatment, or with both enzyme
and plasma treatment for 5 min (composition: 0.1 U ml-1 HRP, 5 mM guaiacol, 50 mM KPi pH
Protein concentration
was determined using the Bradford method. Specific heme content
was set as absorbance at l=403 (see Figure 3) divided by protein concentration
Plasma treatment was performed
with 110 mL HRP (1 kUmL@1) in KPi buffer (100 mm, pH 6.5) without
guaiacol. Activity was measured by diluting the treated samples to
CD spectroscopy of SodA after different durations of plasma treatment. CD spectra of residual SodA were obtained after plasma treatment
Protein concentration after plasma treatment was determined by the Bradford assay.
Absorption spectra of SOD assay solutions after 10 min pre-incubation and before starting the assay reaction.
2,4‐dinitrophenylhydrazine (DNPH), which reacts with carbonyl groups, was used to grossly measure protein oxidation.
