110 μl of buffer were treated for 10 min as described before and H2O2
concentration was measured using the Spectroquant kit. Then, rAaeUPO was added at 40 nM
and incubated for 2 min. For the sample using diluted H2O2, the same concentration of H2O2
was used as had been measured in the plasma-treated samples. ABTS was added as
substrate and activity was measured as described before, substituting sodium acetate buffer
for potassium phosphate buffer (pH 7) and using only the H2O2 already present in the samples.
Activity of rAaeUPO without incubation represents 100%. n=3.