Glutaraldehyde-activated polymethacrylate beads (Relizyme HA403) were incubated
with HRP overnight to yield an immobilized HRP solution corresponding
to 20 UmL@1. For both enzyme formulations, 100 mL was treated
at 13.5 kV and 300 Hz. Free enzyme activity was measured as described
above. Activity assays for immobilized HRP were conducted with constant
shaking to provide sufficient substrate delivery to the macroscopic beads,
using 5 mm guaiacol and 0.25 mm H2O2. The displayed relative activities
were calculated by relating the activities of treated samples to their respective
untreated controls. Data represent means of three replicates.