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Applied Microbiology

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Creative Commons Attribution 4.0 International
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Dielectric barrier discharge plasma treatment affects stability, metal ion coordination, and enzyme activity of bacterial superoxide dismutases

Plasma Medicine

A molecular‐level understanding of the effects of atmospheric‐pressure plasma on biological samples requires knowledge of the effects on proteins. Superoxide dismutases, which detoxify superoxide under oxidative stress conditions, play a key role in bacterial plasma resistance. Investigation of the impact of dielectric barrier discharge (DBD) treatment on purified superoxide dismutases SodA and SodB of Escherichia coli showed that DBD treatment caused a rapid protein degradation, with only 8% of protein remaining after 10 min. The affinity of SodA for the metal cofactor Mn2+ was reduced. Mass spectrometry, in conjunction with coupled‐cluster calculations, revealed that modifications of amino acid residues in the active site can explain the decreased metal affinity and a distortion of the coordination geometry responsible for the activity loss.

iron
manganese
peroxynitrite
plasma medicine
FieldValue
Publisher
Applied Microbiology
Authors
M. Krewig
C. Jung
E. Dobbelstein
B. Schubert
T. Jacob
J. Bandow
Release Date
2020-07-09
Identifier
2a74a58b-0d0f-40ed-a02b-3071d91c6cb8
Permanent Identifier (URI)
https://rdpcidat.rub.de/node/133
Is supplementing
Plasma Processes and Polymers ppap.2020000019 (2020)
Plasma Source Name
Cinogy PlasmaDerm DBD
Plasma Source Application
dermatology
Plasma Source Specification
atmospheric pressure
non-thermal
AC
low frequency
Plasma Source Properties
V(RMS) = 13.5 kV, trigger frequency = 300 Hz, diameter=20 mm
Language
English (United States)
Plasma Source Procedure
Aqueous sample of 40 μl on stainless steel support placed on grounded support. Distance between sample and DBD was 1 mm.
License
Creative Commons Attribution 4.0 International
Plasma Medium Name
Air
Plasma Medium Properties
room temperature. no further control.
Plasma Target Name
aqueous
Contact Name
Bandow, Julia E.
Plasma Target Properties
purified superoxide dismutase. Escherichia coli BW25113, either ∆sodA, ∆sodB, ∆sodC, or ∆sodA∆sodB, taken from KEIO collection. 40 μl sample
Contact Email
julia.bandow@rub.de
Public Access Level
Public
Funding Agency
German Science Foundation (DFG)
Project
SFB 1316
Subproject
B8

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