A maximal estimate of peroxynitrite in plasma‐ treated buffer was calculated on the basis of the assumptions that besides H2O2, peroxynitrite is the only plasma‐generated species interfering with the superoxide dismutase (SOD) activity assay and there are no reactions between the reactive species at the time of the assay. The concentration was calculated on the basis of the activity of a peroxynitrite solution of a defined concentration in the SOD activity assay and the activity of plasma‐treated buffer minus the interfering activity determined for H2O2. The untreated sample could not be used to calculate the ONOO− concentration, as it did not interfere with the SOD activity assay.
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