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S4a Influence of manganese ions on SOD activity assay

Manganese salts (100 μM), untreated SodA, or plasma-treated SodA (10 min) were added to the SOD activity assay with or without adding EDTA (1 mM) during the pre-incubation. To determine the activity of plasma-treated SodA, 5 μg ml-1 of protein as measurement by Bradford assay after plasma treatment were applied in the activity assay. The activity of the plasma-treated SodA (SodA10) was corrected for interference of plasma-generated species in the assay.

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