After removal of the manganese cofactor from untreated or plasma‐treated SodA, the protein was reconstituted by adding different amounts of Mn2+ to the apoSodA. The incorporation of manganese into the protein was observed by measuring the intrinsic tryptophan fluorescence (λex = 280 nm, λem = 340 nm). For calculation of the dissociation constant Kd, the FI values were corrected for the FI of the sample without Mn2+ yielding ∆FI. Then, Each ∆FI was set in relation to the maximally achieved ∆FI giving ∆FI/∆FI_max.