{"help":"Return the metadata of a dataset (package) and its resources. :param id: the id or name of the dataset :type id: string","success":true,"result":[{"id":"2a74a58b-0d0f-40ed-a02b-3071d91c6cb8","name":"dielectric-barrier-discharge-plasma-treatment-affects-stability-metal-ion-coordination-and","title":"Dielectric barrier discharge plasma treatment affects stability, metal ion coordination, and enzyme activity of bacterial superoxide dismutases","author_email":"julia.bandow@rub.de","maintainer":"Research Data Repository","maintainer_email":"achim.vonkeudell@rub.de","license_title":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/","notes":"\u003Cp\u003EA molecular\u2010level understanding of the effects of atmospheric\u2010pressure plasma on biological samples requires knowledge of the effects on proteins. Superoxide dismutases, which detoxify superoxide under oxidative stress conditions, play a key role in bacterial plasma resistance. Investigation of the impact of dielectric barrier discharge (DBD) treatment on purified superoxide dismutases SodA and SodB of \u003Cem\u003EEscherichia coli\u003C\/em\u003E showed that DBD treatment caused a rapid protein degradation, with only 8% of protein remaining after 10 min. The affinity of SodA for the metal cofactor Mn2+ was reduced. Mass spectrometry, in conjunction with coupled\u2010cluster calculations, revealed that modifications of amino acid residues in the active site can explain the decreased metal affinity and a distortion of the coordination geometry responsible for the activity loss.\u003C\/p\u003E\n","url":"https:\/\/rdpcidat.rub.de\/dataset\/dielectric-barrier-discharge-plasma-treatment-affects-stability-metal-ion-coordination-and","state":"Active","log_message":"Update to resource 01b Bacterial growth with paraquat","private":true,"revision_timestamp":"Sun, 03\/21\/2021 - 19:28","metadata_created":"Thu, 07\/09\/2020 - 14:34","metadata_modified":"Sun, 03\/21\/2021 - 19:28","creator_user_id":"f6468e5b-fa18-4bef-a23a-905017123712","type":"Dataset","resources":[{"id":"a93c291f-9376-41d9-b7e6-22bbca317553","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/01a%20SOD%20capacity.csv","description":"\u003Cp\u003EOne hundred micrograms of soluble protein extract of exponentially growing cultures of the wild type and deletion strains were subjected to SOD activity measurements.\u003C\/p\u003E\n","format":"csv","state":"Active","revision_timestamp":"Sun, 03\/21\/2021 - 15:04","name":"01a SOD capacity of E.coli","mimetype":"text\/csv","size":"462 bytes","created":"Thu, 07\/09\/2020 - 14:48","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Sun, 03\/21\/2021 - 15:04"},{"id":"8a1da02c-40ef-4379-b0a7-651f21f0605e","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/01b%20growth%20with%20paraquat.csv","description":"\u003Cp\u003EParaquat sensitivity of SOD\u2010deletion strains was determined in growth experiments. E. coli cells were exposed to paraquat (200 \u03bcM) or left untreated. Final ODs after 16\u2010hr incubation are shown.\u003C\/p\u003E\n","format":"csv","state":"Active","revision_timestamp":"Sun, 03\/21\/2021 - 19:28","name":"01b Bacterial growth with paraquat","mimetype":"text\/csv","size":"361 bytes","created":"Fri, 07\/10\/2020 - 12:54","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Sun, 03\/21\/2021 - 19:28"},{"id":"125dc190-8cc8-4933-a162-b72376df09eb","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/01c%20bacterial%20plasmas%20sensitivity.csv","description":"\u003Cp\u003EPlasma sensitivity of SOD\u2010deletion strains was determined in survival assays. E. coli cells in the mid\u2010log phase were exposed to DBD plasma (1 min), and for each strain, survival was set in relation to the respective untreated controls.\u003C\/p\u003E\n","format":"csv","state":"Active","revision_timestamp":"Sun, 03\/21\/2021 - 19:28","name":"01c Bacterial plasma sensitivity","mimetype":"text\/csv","size":"225 bytes","created":"Fri, 07\/10\/2020 - 13:02","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Sun, 03\/21\/2021 - 19:28"},{"id":"e1a507a4-b774-49fa-904d-64464d14e136","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/02b%20residual%20protein%20activity.csv","description":"\u003Cp\u003EThe activity of residual SodA and SodB (relative specific activity) was determined. Five \u03bcg\/ml of plasma\u2010treated protein was applied in the activity assay, based on the Bradford assay results. Assay results were corrected for interference of plasma-treated buffer.\u003C\/p\u003E\n","format":"csv","state":"Active","revision_timestamp":"Sun, 03\/21\/2021 - 19:27","name":"02b Residual protein activity after plasma treatment","mimetype":"text\/csv","size":"396 bytes","created":"Fri, 07\/10\/2020 - 13:29","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Sun, 03\/21\/2021 - 19:27"},{"id":"5888a6db-b803-46d1-b510-8c85d9792025","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/02c%20protein%20carbonylation.csv","description":"\u003Cp\u003E2,4\u2010dinitrophenylhydrazine (DNPH), which reacts with carbonyl groups, was used to grossly measure protein oxidation. Protein solutions containing 40 \u03bcg of SodA or SodB were exposed to plasma, and the whole sample was subjected to the reaction with DNPH.\u003C\/p\u003E\n","format":"csv","state":"Active","revision_timestamp":"Sun, 03\/21\/2021 - 19:27","name":"02c Protein carbonylation after plasma treatment","mimetype":"text\/csv","size":"588 bytes","created":"Fri, 07\/10\/2020 - 13:35","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Sun, 03\/21\/2021 - 19:27"},{"id":"9ea95d7c-e8c5-481d-8884-4453194b4f2b","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/03%20calculated%20peroxynitrite%20concentration.csv","description":"\u003Cp\u003EA maximal estimate of peroxynitrite in plasma\u2010 treated buffer was calculated on the basis of the assumptions that besides H2O2, peroxynitrite is the only plasma\u2010generated species interfering with the superoxide dismutase (SOD) activity assay and there are no reactions between the reactive species at the time of the assay. The concentration was calculated on the basis of the activity of a peroxynitrite solution of a defined concentration in the SOD activity assay and the activity of plasma\u2010treated buffer minus the interfering activity determined for H2O2. The untreated sample could not be used to calculate the ONOO\u2212 concentration, as it did not interfere with the SOD activity assay.\u003C\/p\u003E\n","format":"csv","state":"Active","revision_timestamp":"Sun, 03\/21\/2021 - 19:27","name":"03 Estimation of plasma-generated peroxynitrite","mimetype":"text\/csv","size":"168 bytes","created":"Fri, 07\/10\/2020 - 13:42","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Sun, 03\/21\/2021 - 19:27"},{"id":"15ec1633-a1cd-4542-a085-dacf2c1518b9","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/05a%20raw%20fluorescence%20intensities_0.csv","description":"\u003Cp\u003EAfter removal of the manganese cofactor from untreated or plasma\u2010treated SodA, the protein was reconstituted by adding different amounts of Mn2+ to the apoSodA. The incorporation of manganese into the protein was observed by measuring the intrinsic tryptophan fluorescence (\u03bbex = 280 nm, \u03bbem = 340 nm). For calculation of the dissociation constant Kd, the FI values were corrected for the FI of the sample without Mn2+ yielding \u2206FI. Then, Each \u2206FI was set in relation to the maximally achieved \u2206FI giving \u2206FI\/\u2206FI_max.\u003C\/p\u003E\n","format":"csv","state":"Active","revision_timestamp":"Sun, 03\/21\/2021 - 19:26","name":"05a Raw tryptophan fluorescence of SodA for Mn-reconstitution","mimetype":"text\/csv","size":"690 bytes","created":"Fri, 07\/10\/2020 - 15:55","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Sun, 03\/21\/2021 - 19:26"},{"id":"6ad1adfe-7ba7-45ac-8acb-87136ccf5473","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/S2a%20influence%20of%20plasma-treated%20buffer.csv","description":"\u003Cp\u003EThe buffer (potassium phosphate, 100 mM, pH 7.5) was treated with the DBD for up to 10 min and different volumes were applied in the SOD activity assay.\u003C\/p\u003E\n","format":"csv","state":"Active","revision_timestamp":"Sun, 03\/21\/2021 - 19:26","name":"S2a SOD-mimicking activity of plasma- treated buffer","mimetype":"text\/csv","size":"569 bytes","created":"Fri, 07\/10\/2020 - 16:02","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Sun, 03\/21\/2021 - 19:26"},{"id":"b91a8248-d812-4536-a8fb-340019dc372e","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/S2b%20influence%20of%20ROS%20in%20enzyme%20assay.csv","description":"\u003Cp\u003EConcentration-dependent activity of different long-living reactive species known to be formed in plasma-treated buffers in the SOD activity assay. ROS were added instead of SODs, giving the indicated final concentrations in the assay solution.\u003C\/p\u003E\n","format":"csv","state":"Active","revision_timestamp":"Sun, 03\/21\/2021 - 19:25","name":"S2b SOD-mimicking activity of ROS in the enzyme assay","mimetype":"text\/csv","size":"521 bytes","created":"Fri, 07\/10\/2020 - 16:09","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Sun, 03\/21\/2021 - 19:25"},{"id":"502e8432-1733-42a7-9cdd-986baca73562","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/S2c%20hydrogen%20peroxide%20generation.csv","description":"\u003Cp\u003EGeneration of hydrogen peroxide in buffer treated with DBD plasma as a function of treatment time determined using the Spectroquant kit (Merck).\u003C\/p\u003E\n","format":"csv","state":"Active","revision_timestamp":"Sun, 03\/21\/2021 - 19:25","name":"S2c generation of Hydrogen peroxide by plasma","mimetype":"text\/csv","size":"177 bytes","created":"Fri, 07\/10\/2020 - 16:12","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Sun, 03\/21\/2021 - 19:25"},{"id":"0f894340-1ea3-409c-9bbd-1591c7175b08","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/S2d%20influence%20of%20catalase%20KatE.csv","description":"\u003Cp\u003ESOD-mimicking activity of plasma-treated buffer (10 min plasma treatment) incubated with and without catalase (5\u00a0\u03bcg\/ml, 10-25 U, bovine liver, Sigma Aldrich) prior to performing the SOD activity assay in the absence if SOD to determine the combined influence of plasma generated H2O\u0022 and ONOO-.\u003C\/p\u003E\n","format":"csv","state":"Active","revision_timestamp":"Sun, 03\/21\/2021 - 19:24","name":"S2d Influence of catalase KatE on the activity of plasma-treated buffer","mimetype":"text\/csv","size":"159 bytes","created":"Fri, 07\/10\/2020 - 16:16","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Sun, 03\/21\/2021 - 19:24"},{"id":"2cca2da9-d03c-4c8b-9df2-79a513673ca6","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/S3%20absorption%20spectra.csv","description":"\u003Cp\u003EAbsorption spectra of SOD assay solutions after 10 min pre-incubation and before starting the assay reaction. NADH, nicotinamide adenine dinucleotide; NBT, nitroblue tetrazolium; PTB, plasma-treated buffer.\u003C\/p\u003E\n","format":"csv","state":"Active","revision_timestamp":"Sun, 03\/21\/2021 - 19:24","name":"S3 Absorption spectra of NBT and NADH with or without plasma-treated buffer (PTB)","mimetype":"text\/csv","size":"18.98 KB","created":"Fri, 07\/10\/2020 - 16:22","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Sun, 03\/21\/2021 - 19:24"},{"id":"ee20427d-a985-493a-ab75-bb6c300c7776","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/02a%20protein%20concentration.csv","description":"\u003Cp\u003EProtein concentration after plasma treatment was determined by the Bradford assay.\u003C\/p\u003E\n","format":"csv","state":"Active","revision_timestamp":"Sun, 03\/21\/2021 - 19:24","name":"02a Protein concentration after plasma treatment","mimetype":"text\/csv","size":"446 bytes","created":"Fri, 07\/10\/2020 - 16:30","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Sun, 03\/21\/2021 - 19:24"},{"id":"d5f0297f-9d2d-4a5d-b21d-27ce10f5b3c4","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/S4a%20influence%20of%20manganese.csv","description":"\u003Cp\u003EManganese salts (100 \u03bcM), untreated SodA, or plasma-treated SodA (10 min) were added to the SOD activity assay with or without adding EDTA (1 mM) during the pre-incubation. To determine the activity of plasma-treated SodA, 5 \u03bcg ml-1 of protein as measurement by Bradford assay after plasma treatment were applied in the activity assay. The activity of the plasma-treated SodA (SodA10) was corrected for interference of plasma-generated species in the assay.\u003C\/p\u003E\n","format":"csv","state":"Active","revision_timestamp":"Sun, 03\/21\/2021 - 19:23","name":"S4a Influence of manganese ions on SOD activity assay","mimetype":"text\/csv","size":"323 bytes","created":"Fri, 07\/10\/2020 - 16:40","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Sun, 03\/21\/2021 - 19:23"},{"id":"ce27aa5e-3049-487d-9dc7-d834c8632bf7","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/S4b%20influence%20of%20iron.csv","description":"\u003Cp\u003ETo determine the influence of free iron ions on the SOD activity assay, the initial slope in the change of absorbance was determined after addition of different concentrations of iron salts to the SOD activity assay in the absence of SODs.\u003C\/p\u003E\n","format":"csv","state":"Active","revision_timestamp":"Sun, 03\/21\/2021 - 19:23","name":"S4b Influence of iron ions on the SOD activity assay","mimetype":"text\/csv","size":"196 bytes","created":"Fri, 07\/10\/2020 - 16:42","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Sun, 03\/21\/2021 - 19:23"},{"id":"17eb1f32-0090-4da3-b519-f8b099db3641","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/S5%20CD%20spectroscopy%20of%20SodA.csv","description":"\u003Cp\u003ECD spectroscopy of SodA after different durations of plasma treatment. CD spectra of residual SodA were obtained after plasma treatment\u003C\/p\u003E\n","format":"csv","state":"Active","revision_timestamp":"Sun, 03\/21\/2021 - 19:22","name":"S5 CD spectroscopy of SodA","mimetype":"text\/csv","size":"109.84 KB","created":"Fri, 07\/10\/2020 - 16:51","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Sun, 03\/21\/2021 - 19:22"}],"tags":[{"id":"c1502f79-1566-44d7-8437-9eb8657ecdc0","vocabulary_id":"2","name":"iron"},{"id":"8d41ddbc-1b40-4bc7-8341-5ae6badbf307","vocabulary_id":"2","name":"manganese"},{"id":"07581cad-692c-49b4-bc4a-710203c80827","vocabulary_id":"2","name":"peroxynitrite"},{"id":"d65fcb1a-2c3b-4490-a6fb-5a8d3d79f7de","vocabulary_id":"2","name":"plasma medicine"}],"groups":[{"description":"","id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","image_display_url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/rublogoweiss_0_1.png","title":"Applied Microbiology","name":"group\/applied-microbiology"}]}]}