Plasma-driven biocatalysis with the capillary plasma jet using CYP152BSβ using complete buffer exchange every 10 min.
The reaction solution containing 5 ml potassium phosphate buffer (100 mmol l-1, pH 7) with 50 mmol l-1 ETBE and 20 mmol l-1 heptanoic acid (as decoy molecule) and was exchanged every 10 min. Product conversion of the substrate ETBE were evaluated every 10 min (a) and product accumulation (b) were evaluated with H2O2, which was supplied by plasma treatment of the reaction solution containing CYP152BSβ immobilized on ReliZyme HA403 M using a capillary plasma jet and 1280 ppm H2O in the feed gas by GC measurement. Plasma treatment was continued for a total of 120 min. Means and standard deviations reflect three experiments.