Plasma-driven biocatalysis (1280 ppm H2O in feed gas) with capillary plasma jet using CYP152BSβ.
Product conversion of the substrate ETBE using direct plasma treatment of CYP152BSβ immobilized on ReliZyme HA403 M beads. Reaction solution contained 5 ml potassium phosphate buffer (100 mmol l-1, pH 7) with 50 mmol l-1 ETBE and 20 mmol l-1 heptanoic acid (as decoy molecule). Plasma treatment was performed with a water concentration of 1280 ppm in the feed gas. Every 5 min, aliquots were withdrawn for product analysis by GC measurement and the reaction volume was topped up with reaction solution to 5 ml. Means and standard deviations reflect three experiments.