Supplementary Figure 1: Spectral analysis of purified CYP152BSb overproduced in LB medium. For cypC overexpression, cultures were inoculated to an OD600 of 0.05 and incubated at 37°C to an OD600 of 0.5-0.6, followed by an induction with IPTG (100 μmol l-1). After 4 h incubation at 30°C, the cells were harvested and used for protein purification. Absorption spectra were recorded using 12 μmol l- 1 CYP152BSb in potassium phosphate buffer (100 mmol l-1, pH 7). Buffer served as blank. R/z value was calculated by relating absorption of the soret peak (at 420nm) to absorption at 280nm. Representative data of three independent replicates is displayed.