Spectral analysis of purified CYP152BSβ before and after reconstitution.
Absorption spectra were recorded using 12 µmol l-1 CYP152BSβ in potassium phosphate buffer (100 mmol l-1, pH 7). Buffer served as blank. R/z values were calculated by relating absorption of the soret peak (at 420 nm) to absorption at 280 nm. Data shown are representative of three independent replicates.