Figure 8b hydroxyl radicals

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Relative chaperone activity at different hydrogen peroxide concentrations is displayed. The Fenton reaction was used to generate hydroxyl radicals. 0.5 mM FeSO4 was incubated together with up to 10 mM H2O2 and Hsp33 for 1 h. Formation of hydroxyl radicals was monitored using terephthalic acid, which reacts with hydroxyl radicals to HTA leading to a fluorescence signal (λex= 315 nm;λem= 425 nm). The incubation of Hsp33 with hydroxyl radicals was carried out at room temperature.

Figure 8b.xlsx

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Figure 1a Survival Rates
Figure 1b Survival longer treatment
Figure 2a Plasma stability Hsp33
Figure 2b Hsp33 Degradation
Figure 3a Plasma-induced Hsp33 activation
Figure 3b CD spectra Hsp33
Figure 4a Hsp33 Carbonylation
Figure 4b Hsp33 Tryptophan fluorescence
Figure 4c,d Hsp33 zincon assay
Figure 5a,b Superoxide
Figure 5c,d singlet oxygen
Figure 5e,f atomic oxygen
Figure 6 Hsp33 µAPPJ activation
Figure 7 ozone degradation
Figure 8a peroxynitrite
Figure 8b hydroxyl radicals
Figure 9 Scavenger

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timestamp	Dec 06, 2023