Figure 3a Plasma-induced Hsp33 activation

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Determination of relative chaperone activity and thiol oxidation after different plasma treatment times. The relative chaperone activity was determined by a citrate synthase-based assay, to which aggregate-free Hsp33 was added. Activity of Hsp33red was set to 0%, activity of Hsp33HOCl was set to 100%. The thiol oxidation state was determined by incubation of plasma-treated Hsp33 with Ellman’s reagent. Afterwards, absorption at412 nm was measured.

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Figure 1a Survival Rates
Figure 1b Survival longer treatment
Figure 2a Plasma stability Hsp33
Figure 2b Hsp33 Degradation
Figure 3a Plasma-induced Hsp33 activation
Figure 3b CD spectra Hsp33
Figure 4a Hsp33 Carbonylation
Figure 4b Hsp33 Tryptophan fluorescence
Figure 4c,d Hsp33 zincon assay
Figure 5a,b Superoxide
Figure 5c,d singlet oxygen
Figure 5e,f atomic oxygen
Figure 6 Hsp33 µAPPJ activation
Figure 7 ozone degradation
Figure 8a peroxynitrite
Figure 8b hydroxyl radicals
Figure 9 Scavenger

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timestamp	Dec 06, 2023