enzyme assay

In
order to extract the heme from HRP, acidified enzyme solution was incubated with an excess

Supernatant
of the reaction vial, that is, buffer without enzyme, was treated as mentioned
before and added back into the container. This was repeated for

Glutaraldehyde-activated polymethacrylate beads (Relizyme HA403) were incubated
with HRP overnight to yield an immobilized HRP solution corresponding

HRP was plasma-treated at 10 UmL@1 for 1 min and activity was
subsequently measured ex situ as described above. Untreated samples were

Plasma treatment was performed
with 110 mL HRP (1 kUmL@1) in KPi buffer (100 mm, pH 6.5) without
guaiacol. Activity was measured by diluting the treated samples to

To determine the influence of free iron ions on the SOD activity assay, the initial slope in the change of absorbance was determined after addition of different concentrations of iron salts to the

Manganese salts (100 μM), untreated SodA, or plasma-treated SodA (10 min) were added to the SOD activity assay with or without adding EDTA (1 mM) during the pre-incubation.

SOD-mimicking activity of plasma-treated buffer (10 min plasma treatment) incubated with and without catalase (5 μg/ml, 10-25 U, bovine liver, Sigma Aldrich) prior to performing the SOD activity as

Concentration-dependent activity of different long-living reactive species known to be formed in plasma-treated buffers in the SOD activity assay.

The buffer (potassium phosphate, 100 mM, pH 7.5) was treated with the DBD for up to 10 min and different volumes were applied in the SOD activity assay.