Plasma-mediated protein detachment from the carrier material were investigated using Ni-NTA agarose, to which enzyme is bound non-covalently. Proteins VCPO, LdhA, LacZ, or GapA were immobilized on Ni-NTA agarose and treated with plasma or left untreated. After 300 s of plasma treatment, the amount of protein in the supernatant was determined using a Bradford assay and compared to protein concentrations in the untreated control.