Substrate (ABTS) conversion performing plasma-driven biocatalysis using immobilized rAaeUPO with npMWPT. (a) Conversion of ABTS using immobilized rAaeUPO was tested by transferring 100 mg protein-loaded beads to a rotating bed reactor, which was placed in a vessel filled with 1 ml ABTS (5 mmol l-1) dissolved in 100 mmol l-1 citrate buffer (pH 5). Plasma-treated water was added at a flow rate of 2.5 ml min 1 for 2 min, while the absorbance was measured every 30 s at 405 nm. To prove the reusability of the rAaeUPO-loaded beads, the whole reaction solution was withdrawn from the vessel and the reaction cycle was repeated (total cycle amount of five). The total product amount was calculated under consideration of the introduced dilution factor and based on the millimolar extinction coefficient (ε = 36.8 mmol l−1 cm−1). (b) Accumulation of formed product (ABTS* in µmol) over the total run time of 600 s in five reaction cycles. Means and standard deviations reflect three independent experiments. Standard deviations that are not visible were below 0.02 µmol (a) or 0.1 µmol (b).