Conversion of ethylbenzene with immobilized rAaeUPO was analyzed by loading 200 mg enzyme-loaded beads into a rotating bed reactor. The flow of substrate (100 mmol l-1 ethylbenzene in 100 mmol l-1 potassium phosphate buffer, pH 7) was combined with the continuous flow of the plasma-treated water (total flow rate of 5 ml min 1) and continuously supplied to the reaction vessel with the rotating bed reactor for a total run time of 30 min. Every 45 s, samples of 3.75 ml were withdrawn to prevent overflowing of the reaction vessel, while four samples were pooled in one reaction tube (total volume of 15 ml). (a) To analyze product formation in the respective 3 min time frame, 150 µl sample was withdrawn for GC measurement. (b) Accumulation of (R)-1-PhOl performing plasma-driven biocatalysis calculated by addition of all measured (R)-1-PhOl amounts during the 30 min biocatalysis. Means and standard deviations represent three independent experiments.