Influence of cysteine over-production. (A) Dependency of the plasma survival rate on the intracellular cysteine concentration in the strain E. coli pCA24N::cysE (blue). Amounts of IPTG for induction [µM] of cysE are indicated next to each data point. Data for E. coli wild type (without the plasmid) is given as gray triangle. (B) Enzyme activity of malate dehydrogenase (MDH), aconitase (ACN), fumarase (FUM), and succinate dehydrogenase (SDH) after µAPPJ treatment. E. coli pCA24N::cysE induced with 100 µM IPTG was left untreated (gray, set to 1.0), exposed to gas flow (blue), or treated with plasma (orange). Residual enzyme activity was determined either directly after treatment (t0, dark bars) or after incubation at 37°C for 20 min (t20, light bars). Averages and standard deviations represent three independent biological replicates.