Residual activities of rAaeUPO on ReliZyme beads after 80 min plasma-driven biocatalysis with the capillary plasma jet. Biocatalysis was performed with the capillary plasma jet and a water concentration of 6400 ppm in the feed gas at 6 W plasma power for a run time of 80 min. After biocatalysis, enzyme-loaded beads were recovered and washed thrice with potassium phosphate buffer (100 mM, pH 7). Enzyme activity was determined using 2.5 mM 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 1 mM H2O2 and 50 mM citrate. Samples were shaken during turnover to ensure sufficient substrate supply. Every two minutes in a total of ten minutes reaction time, aliquots of 100 μl were removed and measured at 405 nm using a microplate reader (Biotek Epoch). Enzyme activity was calculated based on the linear slope of the kinetic. Means and standard deviations reflect three experiments.