{"help":"Return the metadata of a dataset (package) and its resources. :param id: the id or name of the dataset :type id: string","success":true,"result":[{"id":"ca06a39f-b811-4552-b1fe-ab9f2abe87b0","name":"cold-atmospheric-pressure-plasma-generated-species-superoxide-singlet-oxygen-and-atomic","title":"The cold atmospheric pressure plasma-generated species superoxide, singlet oxygen and atomic oxygen activate the molecular chaperone Hsp33","author_email":"julia.bandow@rub.de","maintainer":"Research Data Repository","maintainer_email":"achim.vonkeudell@rub.de","license_title":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/","notes":"\u003Cp\u003ECold atmospheric pressure plasmas are used for surface decontamination or disinfection, e.g. in clinical settings. Protein aggregation has been shown to significantly contribute to the antibacterial mechanisms of plasma. To investigate the potential role of the redox-activated zinc-binding chaperone Hsp33 in preventing protein aggregation and thus mediating plasma resistance, we compared the plasma sensitivity of wild-type E. coli to that of an hslO deletion mutant lacking Hsp33 as well as an over-producing strain. Over-production of Hsp33 increased plasma survival rates above wild-type levels. Hsp33 was previously shown to be activated by plasma in vitro. For the PlasmaDerm source applied in dermatology, reversible activation of Hsp33 was confirmed. Thiol oxidation and Hsp33 unfolding, both crucial for Hsp33 activation, occurred during plasma treatment. After prolonged plasma exposure, however, unspecific protein oxidation was detected, the ability of Hsp33 to bind zinc ions was decreased without direct modifications of the zinc-binding motif, and the protein was inactivated. To identify chemical species of potential relevance for plasma-induced Hsp33 activation, reactive oxygen species were tested for their ability to activate Hsp33 in vitro. Superoxide, singlet oxygen and potentially atomic oxygen activate Hsp33, while no evidence was found for activation by ozone, peroxynitrite or hydroxyl radicals.\u003C\/p\u003E\n","url":"https:\/\/rdpcidat.rub.de\/dataset\/cold-atmospheric-pressure-plasma-generated-species-superoxide-singlet-oxygen-and-atomic","state":"Active","log_message":"Edited by kd.","private":true,"revision_timestamp":"Sun, 04\/28\/2024 - 11:29","metadata_created":"Wed, 12\/06\/2023 - 13:14","metadata_modified":"Sun, 04\/28\/2024 - 11:29","creator_user_id":"fbaa0bb1-6827-46c5-a646-bca2c4ce442a","type":"Dataset","resources":[{"id":"e2c08c02-1f21-40fb-8142-dae06fe261c0","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/Figure%201a.xlsx","description":"\u003Cp\u003ERelevance of hslO for plasma resistance. Survival rates after 60 s plasma treatment of E. coli wild-type BW25113, the deletion strain \u0394hslO (both with or without the empty vector pCA24N) and the complemented strain \u0394hslO+ pCA24N::hslO induced with different IPTG concentrations. Relative signal intensities monitored in western blots for the IPTG-induced complemented strains are displayed in green.\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Thu, 04\/25\/2024 - 11:10","name":"Figure 1a Survival Rates","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"56.04 KB","created":"Wed, 12\/06\/2023 - 13:15","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Thu, 04\/25\/2024 - 11:10"},{"id":"eee53cfd-09de-4635-9dee-ebbc8d023b6c","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/Figure%201b.xlsx","description":"\u003Cp\u003ESurvival of E.  coli wild-type and the complemented strain \u0394hslO+ pCA24N::hslO induced with 1000 \u03bcM IPTG at different plasma treatment times.\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Thu, 04\/25\/2024 - 11:10","name":"Figure 1b Survival longer treatment","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"33.01 KB","created":"Wed, 12\/06\/2023 - 13:16","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Thu, 04\/25\/2024 - 11:10"},{"id":"94993fbc-f842-4407-aec4-1f45d4f58d04","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/Figure%202a.xlsx","description":"\u003Cp\u003EPlasma stability of Hsp33. Protein concentration of Hsp33 as a function of different plasma treatment times. 1 mg ml\u22121 Hsp33 was treated with plasma for indicated times and Bradford reagent was used to determine the protein concentration of the treated solutions. Concentration of an untreated sample was set to 100%.\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Thu, 04\/25\/2024 - 11:10","name":"Figure 2a Plasma stability Hsp33","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"202.25 KB","created":"Wed, 12\/06\/2023 - 13:18","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Thu, 04\/25\/2024 - 11:10"},{"id":"42df878f-2c1b-4a30-9b9c-7a524c2877ac","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/Figure%202b.xlsx","description":"\u003Cp\u003ETerminal amino functions as a function of different plasma treatment times were determined using the ninhydrin assay. After treatment of 1 mg ml\u22121 Hsp33 with plasma ninhydrin was added, which reacts with terminal amino functions resulting in an increased absorption at 575 nm. As a control, acidic hydrolysis of Hsp33 was performed to obtain signal intensities for maximal degradation.\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Thu, 04\/25\/2024 - 11:10","name":"Figure 2b Hsp33 Degradation","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"63.15 KB","created":"Wed, 12\/06\/2023 - 13:19","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Thu, 04\/25\/2024 - 11:10"},{"id":"57fab6fb-5842-446a-b08f-503afe546a09","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/Figure%203a.xlsx","description":"\u003Cp\u003EDetermination of relative chaperone activity and thiol oxidation after different plasma treatment times. The relative chaperone activity was determined by a citrate synthase-based assay, to which aggregate-free Hsp33 was added. Activity of Hsp33red was set to 0%, activity of Hsp33HOCl was set to 100%. The thiol oxidation state was determined by incubation of plasma-treated Hsp33 with Ellman\u2019s reagent. Afterwards, absorption at412 nm was measured.\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Thu, 04\/25\/2024 - 11:10","name":"Figure 3a Plasma-induced Hsp33 activation","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"96.88 KB","created":"Wed, 12\/06\/2023 - 13:20","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Thu, 04\/25\/2024 - 11:10"},{"id":"4426b44e-22a6-49b9-8316-48351f12f9fc","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/Figure%203b.xlsx","description":"\u003Cp\u003EFar-UV CD spectra of Hsp33red, Hsp33HOCl, and plasma treated Hsp33. Hsp33 was treated with plasma for 30 (P30), 60 (P60), 120 (P120), and 300 s (P300) and measured immediately.\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Thu, 04\/25\/2024 - 11:09","name":"Figure 3b CD spectra Hsp33","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"759.05 KB","created":"Wed, 12\/06\/2023 - 13:21","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Thu, 04\/25\/2024 - 11:09"},{"id":"ae025cdd-eaf8-4aa4-9c26-f0c9ace32600","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/Figure%204a.xlsx","description":"\u003Cp\u003ECarbonylation level of plasma-treated Hsp33 as measured mixing DNPH with Hsp33. The absorption at 405 nm was measured and corrected for untreated Hsp33.\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Thu, 04\/25\/2024 - 11:09","name":"Figure 4a Hsp33 Carbonylation","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"165.44 KB","created":"Wed, 12\/06\/2023 - 13:22","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Thu, 04\/25\/2024 - 11:09"},{"id":"9081b336-38b0-4468-88d6-7a36688a67d0","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/Figure%204b.xlsx","description":"\u003Cp\u003EZinc-induced conformational changes of Hsp33HOCl or plasma-oxidized Hsp33 (P30, P60, P120) by following the changes in tryptophan fluorescence at 340 nm.\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Thu, 04\/25\/2024 - 11:10","name":"Figure 4b Hsp33 Tryptophan fluorescence ","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"250.74 KB","created":"Wed, 12\/06\/2023 - 13:23","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Thu, 04\/25\/2024 - 11:10"},{"id":"9fb67a77-77e0-4d50-9fbf-37ac268ae370","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/Figure%204c.xlsx","description":"\u003Cp\u003EZinc binding ability of chemically (Hsp33HOCl) or plasma-oxidized (P30, P60, P120) Hsp33 using the zincon assay. Zinc-binding ability of Hsp33 using the zincon assay. Reduced (Hsp33red), chemically oxidized (Hsp33HOCl) and plasma-treated (P30,P60, P120) Hsp33 were investigated. Proteins CnoX and RidA served as controls for unspecific ZnCl2 binding.\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Thu, 04\/25\/2024 - 11:10","name":"Figure 4c,d Hsp33 zincon assay","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"31.59 KB","created":"Wed, 12\/06\/2023 - 13:25","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Thu, 04\/25\/2024 - 11:10"},{"id":"b59b16c7-c33a-4dc6-b17c-d3cf461d5fd1","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/Figure%205ab.xlsx","description":"\u003Cp\u003EHsp33 activation by superoxide. Relative chaperone activity and thiol oxidation upon addition of different amounts of NADH (80, 400 and 800 \u03bcM). Incubation of Hsp33 with the reaction mixture (NBT, NADH and PMS) was carried out at room temperature or 43\u00b0C.\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Thu, 04\/25\/2024 - 11:10","name":"Figure 5a,b Superoxide ","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"107.8 KB","created":"Wed, 12\/06\/2023 - 13:38","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Thu, 04\/25\/2024 - 11:10"},{"id":"20fbca43-d1b2-4ef2-a9e1-3c2dcf63192b","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/Figure%205%20cd.xlsx","description":"\u003Cp\u003EHsp33 activation by singlet oxygen. Relative chaperone activity and thiol oxidation at different excitation times of methylene blue, leading to singlet oxygen formation.The incubation of Hsp33 with singlet oxygen was carried out at room temperature or 43\u00b0C.\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Thu, 04\/25\/2024 - 11:10","name":"Figure 5c,d singlet oxygen","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"76.65 KB","created":"Wed, 12\/06\/2023 - 13:39","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Thu, 04\/25\/2024 - 11:10"},{"id":"1adb1075-49c5-4bc2-9bb0-6c4349ee877b","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/Figure%205ef.xlsx","description":"\u003Cp\u003EHsp33 was treated for 30 s with the effluent of the plasma jet, while the oxygen content was varied (0\u20131% O2). Variations in oxygen admixture lead to production of different amounts of atomic oxygen. Afterwards, chaperone activity and thiol oxidation were determined.\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Thu, 04\/25\/2024 - 11:10","name":"Figure 5e,f atomic oxygen","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"70.19 KB","created":"Wed, 12\/06\/2023 - 13:39","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Thu, 04\/25\/2024 - 11:10"},{"id":"fd37d613-d9dd-42e1-b4d2-6def834b2abb","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/Figure%206.xlsx","description":"\u003Cp\u003EDistance-dependent activation of Hsp33 activation using the \u03bcAPPJ plasma jet. Hsp33 was treated for 30 s with the effluent of the plasma jet, while the distance was varied (0\u201320 mm). This variation of the distance leads to treatment with different densities of atomic oxygen and ozone. Afterwards, chaperone activity was measured.\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Thu, 04\/25\/2024 - 11:10","name":"Figure 6 Hsp33 \u00b5APPJ activation","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"43.11 KB","created":"Wed, 12\/06\/2023 - 13:43","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Thu, 04\/25\/2024 - 11:10"},{"id":"216ae388-1dc5-4229-8191-0682ae095837","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/Figure%207.xlsx","description":"\u003Cp\u003EOzone-dependent Hsp33 degradation. Hsp33 was treated for 60 s with the effluent of the plasma jet at either 3 or 20 mm distance from the end of the nozzle of the jet. Afterwards, ninhydrin was added to quantify terminal aminofunctions.\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Thu, 04\/25\/2024 - 11:10","name":"Figure 7 ozone degradation","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"28.89 KB","created":"Wed, 12\/06\/2023 - 13:50","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Thu, 04\/25\/2024 - 11:10"},{"id":"193c424d-67ef-4ba1-a0ad-c73f33b1d943","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/Figure%208a.xlsx","description":"\u003Cp\u003EInvestigation of Hsp33 activation upon treatment with peroxynitrite. Relative chaperone activities at different amounts of peroxynitrite added are displayed. Incubation of Hsp33 with peroxynitrite was carried out at room temperature or 43\u00b0C.\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Thu, 04\/25\/2024 - 11:10","name":"Figure 8a peroxynitrite ","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"77.73 KB","created":"Wed, 12\/06\/2023 - 14:01","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Thu, 04\/25\/2024 - 11:10"},{"id":"4d6657d9-8e5e-4918-9588-0b13aacc435a","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/Figure%208b.xlsx","description":"\u003Cp\u003ERelative chaperone activity at different hydrogen peroxide concentrations is displayed. The Fenton reaction was used to generate hydroxyl radicals. 0.5 mM FeSO4 was incubated together with up to 10 mM H2O2 and Hsp33 for 1 h. Formation of hydroxyl radicals was monitored using terephthalic acid, which reacts with hydroxyl radicals to HTA leading to a fluorescence signal (\u03bbex= 315 nm;\u03bbem= 425 nm). The incubation of Hsp33 with hydroxyl radicals was carried out at room temperature.\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Thu, 04\/25\/2024 - 11:21","name":"Figure 8b hydroxyl radicals","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"40.12 KB","created":"Wed, 12\/06\/2023 - 14:23","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Thu, 04\/25\/2024 - 11:21"},{"id":"b141d9b4-0785-4b9c-8a40-299a6637a85d","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/Figure%209%20.xlsx","description":"\u003Cp\u003EPlasma-induced Hsp33 activation in the presence of scavengers. Hsp33 was treated for 120 s in presence of different concentrations of L-histidine and MnTBAP. Activity without scavenger addition was set to 100%. Combination of both scavengers was also conducted with 10 mM of each scavenger.\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Thu, 04\/25\/2024 - 11:21","name":"Figure 9 Scavenger ","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"94.33 KB","created":"Wed, 12\/06\/2023 - 14:24","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Thu, 04\/25\/2024 - 11:21"}],"tags":[{"id":"67599de3-0a02-43ef-9718-31c000fc35bf","vocabulary_id":"2","name":"plasma resistance"},{"id":"3c71f23f-38fe-4d22-adb0-8cb0b52679c0","vocabulary_id":"2","name":"plasma protein interaction"},{"id":"f72c48c5-be25-4e21-8f01-f7883d55a825","vocabulary_id":"2","name":"aggregation"},{"id":"b2d0ae85-af33-45c4-aa75-dcabe23467f3","vocabulary_id":"2","name":"zinc coordination"}],"groups":[{"description":"","id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","image_display_url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/rublogoweiss_0_1.png","title":"Applied Microbiology","name":"group\/applied-microbiology"}]}]}