{"help":"Return the metadata of a dataset (package) and its resources. :param id: the id or name of the dataset :type id: string","success":true,"result":[{"id":"1e969046-ba6d-49f9-9c41-112f2287c094","name":"atmospheric-pressure-capillary-plasma-jet-well-suited-supply-h2o2-plasma-driven-biocatalysis","title":"The atmospheric pressure capillary plasma jet is well-suited to supply H2O2 for plasma-driven biocatalysis","author_email":"julia.bandow@rub.de","maintainer":"Research Data Repository","maintainer_email":"achim.vonkeudell@rub.de","license_title":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/","notes":"\u003Cp\u003EPlasma-generated H2O2 can be used to fuel biocatalytic reactions that require H2O2 as co-substrate such as the conversion of ethylbenzene to (R)-1-phenylethanol ((R)-1-PhOl) catalyzed by unspecific peroxygenase from Agrocybe aegerita (rAaeUPO). Immobilization was recently shown to protect biocatalysts from inactivation by highly reactive plasma-produced species, however, H2O2 supply by the employed plasma sources (\u00b5APPJ and DBD) was limiting for rAaeUPO performance. In this study we evaluated a recently introduced capillary plasma jet for suitability to supply H2O2 in situ. H2O2 production was modulated by varying the water concentration in the feed gas, providing a greater operating window for applications in plasma-driven biocatalysis. In a static system after 80 min of biocatalysis, a turnover number of 44,199 mol(R)-1-PhOl mol-1rAaeUPO was achieved without significant enzyme inactivation. By exchanging the reaction solution every 5 min, a total product yield of 122 \u00b5mol (R)-1-PhOl was achieved in 700 min run time, resulting in a total turnover number of 174,209 mol(R)-1-PhOl mol-1rAaeUPO. We conclude that the capillary plasma jet, due to its flexibility regarding feed gas, admixtures, and power input, is well-suited for in situ H2O2 generation for plasma-driven biocatalysis tailoring to enzymes with high H2O2 turnover.\u003C\/p\u003E\n","url":"https:\/\/rdpcidat.rub.de\/dataset\/atmospheric-pressure-capillary-plasma-jet-well-suited-supply-h2o2-plasma-driven-biocatalysis","state":"Active","log_message":"Edited by kd.","private":true,"revision_timestamp":"Tue, 06\/10\/2025 - 13:30","metadata_created":"Tue, 02\/04\/2025 - 13:56","metadata_modified":"Tue, 06\/10\/2025 - 13:30","creator_user_id":"fbaa0bb1-6827-46c5-a646-bca2c4ce442a","type":"Dataset","resources":[{"id":"84b1e6f0-3c6f-4325-8715-73bb65ea2739","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/Figure2_H2O2.xlsx","description":"\u003Cp\u003ETime-dependent H2O2 production.\u003Cbr \/\u003E\nPotassium phosphate buffer (5 ml, 100 mM, pH 7) was treated either with the capillary plasma jet (CPJ) and 6400 ppm water in the feed gas or the microscale atmospheric pressure plasma jet (\u00b5APPJ) and 5750 ppm water in the feed gas for up to 40 min. The distances between the nozzles of the jets and the samples were approx. 16 mm (CPJ) and 6 mm (\u00b5APPJ). Means and standard deviations reflect three experiments. Standard deviations that are not visible were below 0.1 mM.\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Thu, 02\/06\/2025 - 10:25","name":"Figure2","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"45.6 KB","created":"Tue, 02\/04\/2025 - 13:59","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Thu, 02\/06\/2025 - 10:25"},{"id":"7b859193-201d-447c-89bb-2bc43ba7bc92","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/Figure3_H2O2.xlsx","description":"\u003Cp\u003EH2O2 production at different water concentrations in the feed gas.\u003Cbr \/\u003E\nThe feed gas of the capillary jet was (partially) routed through a cooled water-containing bubbler (in %) resulting in different concentrations of water (in ppm) in the feed gas. Potassium phosphate buffer (5 ml, 100 mM, pH 7) was treated for 10 min and the H2O2 concentration (in mM) was determined consecutively. Means and standard deviations represent three experiments shown. Standard deviations that are not visible were below 0.01 mM.\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Thu, 02\/06\/2025 - 10:25","name":"Figure3","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"36.16 KB","created":"Tue, 02\/04\/2025 - 14:00","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Thu, 02\/06\/2025 - 10:25"},{"id":"3f8c202f-3085-41d8-b9c1-9a4842fe51a3","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/Figure4_Relizyme_40.xlsx","description":"\u003Cp\u003EPlasma-driven biocatalysis with the capillary plasma jet using r\u003Cem\u003EAae\u003C\/em\u003EUPO immobilized on ReliZyme beads.\u003Cbr \/\u003E\nConversion of the substrate ETBE utilized H2O2 from direct plasma treatment of the r\u003Cem\u003EAae\u003C\/em\u003EUPO immobilized on ReliZyme HA403 M or EA403 M. Reaction solution contained 5 ml potassium phosphate buffer (100 mM, pH 7) with 50 mM ETBE. Plasma treatment was performed using the capillary plasma jet as described above, with a water concentration of 6400 ppm in the feed gas at 6 W plasma power. Every 5 min, aliquots were withdrawn for product analysis by GC. Means and standard deviations reflect three experiments.\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Thu, 02\/06\/2025 - 10:25","name":"Figure4","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"28.43 KB","created":"Tue, 02\/04\/2025 - 14:02","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Thu, 02\/06\/2025 - 10:25"},{"id":"f3c3e869-c4cb-496b-b62d-acb755592c9a","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/Figure5_Purolite_40.xlsx","description":"\u003Cp\u003EPlasma-driven biocatalysis with the capillary plasma jet and r\u003Cem\u003EAae\u003C\/em\u003EUPO immobilized on Purolite beads.\u003Cbr \/\u003E\nThe substrate ETBE was converted by r\u003Cem\u003EAae\u003C\/em\u003EUPO immobilized on Purolite ECR8309F and ECR8285. The reaction solution contained 5 ml potassium phosphate buffer (100 mM, pH 7) with 50 mM ETBE. H2O2 was generated by plasma treatment with the capillary plasma jet and a water concentration of 6400 ppm in the feed gas at 6 W plasma power. Every 5 min aliquots were withdrawn for product analysis by GC. Means and standard deviations represent three experiments.\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Thu, 02\/06\/2025 - 10:25","name":"Figure5","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"27.28 KB","created":"Tue, 02\/04\/2025 - 14:03","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Thu, 02\/06\/2025 - 10:25"},{"id":"b9a93d1d-5ce7-4971-acf0-6bbc2dc05c61","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/Figure6_Relizyme_80.xlsx","description":"\u003Cp\u003EProlonged plasma-driven biocatalysis with capillary plasma jet.\u003Cbr \/\u003E\nConversion of the substrate ETBE and H2O2 from plasma treatment by r\u003Cem\u003EAae\u003C\/em\u003EUPO immobilized on ReliZyme HA403 M and EA403 M was performed as described above, with the capillary plasma jet and a water concentration of 6400 ppm in the feed gas at 6 W plasma power. Every 5 min aliquots were withdrawn for product quantification by GC. Means and standard deviations reflect three experiments.\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Thu, 02\/06\/2025 - 10:25","name":"Figure6","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"62.35 KB","created":"Tue, 02\/04\/2025 - 14:03","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Thu, 02\/06\/2025 - 10:25"},{"id":"2d83d0c5-1227-4f51-8896-5829bf5e81ae","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/Figure7%2B8.xlsx","description":"\u003Cp\u003EProduct formation per minute in long-term experiments with frequent exchange of reaction solutions.\u003Cbr \/\u003E\nActivity of r\u003Cem\u003EAae\u003C\/em\u003EUPO immobilized on HA403 M beads is shown as a function of plasma-driven biocatalysis run time. Every 10 or 5 min, the complete reaction solution was exchanged, and product formation was analyzed by GC measurement. Either 1280 ppm or 6400 ppm H2O were added to the feed gas to modulate H2O2 generation. Means reflect two experiments. A detailed presentation including standard deviations for each experimental condition is provided in the supplements (Supplementary Figures 6-8).\u003C\/p\u003E\n\u003Cp\u003ETotal product accumulation in the long-term experiment over 5 or 10 min cycles.\u003Cbr \/\u003E\nAccumulation of generated product (\u003Cem\u003ER\u003C\/em\u003E)-1-PhOl produced by r\u003Cem\u003EAae\u003C\/em\u003EUPO immobilized on HA403 M beads after different plasma-driven biocatalysis run times. Either 6400 ppm or 1280 ppm H2O were added to the feed gas for H2O2 generation. Every 10 or 5 min, the complete reaction solution was exchanged, and product formation was analyzed by GC measurement. Means and standard deviations representing two experiments are shown. Standard deviations that are not visible were below 2 \u00b5mol.\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Thu, 02\/06\/2025 - 10:25","name":"Figures7+8","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"133.2 KB","created":"Tue, 02\/04\/2025 - 14:04","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Thu, 02\/06\/2025 - 10:25"},{"id":"5505ddd5-4720-42cb-a1b5-80413e9bb466","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/SupplFigures1_2_3.xlsx","description":"\u003Cp\u003EPlasma-driven biocatalysis with the capillary plasma jet using\u003Cbr \/\u003E\nr\u003Cem\u003EAae\u003C\/em\u003EUPO immobilized on ReliZyme EP403 M beads. Conversion of the substrate ETBE utilized H2O2 from direct plasma treatment of the r\u003Cem\u003EAae\u003C\/em\u003EUPO immobilized on ReliZyme EP403 M. Reaction solution contained 5 ml potassium phosphate buffer (100 mM, pH 7) with 50 mM ETBE. Plasma treatment was performed using the capillary plasma jet as described above, with a water concentration of 6400 ppm in the feed gas at 6 W plasma power. Every 10 min, aliquots were withdrawn for product analysis by GC. Means and standard deviations reflect three experiments.\u003Cbr \/\u003E\nPlasma-driven biocatalysis with the capillary plasma jet using r\u003Cem\u003EAae\u003C\/em\u003EUPO immobilized on ReliZyme HFA403 M beads. Conversion of the substrate ETBE utilized H2O2 from direct plasma treatment of the rAaeUPO immobilized on ReliZyme HFA403 M. Reaction solution contained 5 ml potassium phosphate buffer (100 mM, pH 7) with 50 mM ETBE. Plasma treatment was performed using the capillary plasma jet as described above, with a water concentration of 6400 ppm in the feed gas at 6 W plasma power. Every 10 min, aliquots were withdrawn for product analysis by GC. Means and standard deviations represent three experiments.\u003Cbr \/\u003E\nPlasma-driven biocatalysis with the capillary plasma jet using rAaeUPO immobilized on ReliZyme BU403 M beads. Conversion of the substrate ETBE utilized H2O2 from direct plasma treatment of the rAaeUPO immobilized on ReliZyme BU403 M. Reaction solution contained 5 ml potassium phosphate buffer (100 mM, pH 7) with 50 mM ETBE. Plasma treatment was performed using the capillary plasma jet as described above, with a water concentration of 6400 ppm in the feed gas at 6 W plasma power. Every 10 min, aliquots were withdrawn for product analysis by GC. Means and standard deviations reflect three experiments.\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Thu, 02\/06\/2025 - 10:17","name":"Supplementary Figures1-3","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"41.73 KB","created":"Tue, 02\/04\/2025 - 14:06","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Thu, 02\/06\/2025 - 10:17"},{"id":"f408b876-3793-47b4-84e7-23b98f094309","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/SupplFigure4_residual_act_40.xlsx","description":"\u003Cp\u003EResidual activity of r\u003Cem\u003EAae\u003C\/em\u003EUPO on ReliZyme (HA403M, EA403 M) and Purolite (ECR8309F and ECR8285) beads after 40 min plasma-driven biocatalysis. After biocatalysis, enzyme-loaded beads were recovered and washed thrice with potassium phosphate buffer (100 mM, pH 7). Enzyme activity was determined using 2.5 mM 2,2\u2019-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 1 mM H2O2 and 50 mM citrate. Samples were shaken during turnover to ensure sufficient substrate supply. Every two minutes in a total of ten minutes reaction time, aliquots of 100 \u03bcl were withdrawn and measured at 405 nm using a microplate reader (Biotek Epoch). Enzyme activity was calculated based on the linear slope of the kinetic. Means and standard deviations represent three experiments.\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Thu, 02\/06\/2025 - 10:16","name":"Supplementary Figure4","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"46.58 KB","created":"Tue, 02\/04\/2025 - 14:07","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Thu, 02\/06\/2025 - 10:16"},{"id":"4a95de2d-8d7a-4c94-a8a1-9aa8f2886b19","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/SupplFigure5_residual_act_80.xlsx","description":"\u003Cp\u003EResidual activities of r\u003Cem\u003EAae\u003C\/em\u003EUPO on ReliZyme beads after 80 min plasma-driven biocatalysis with the capillary plasma jet. Biocatalysis was performed with the capillary plasma jet and a water concentration of 6400 ppm in the feed gas at 6 W plasma power for a run time of 80 min. After biocatalysis, enzyme-loaded beads were recovered and washed thrice with potassium phosphate buffer (100 mM, pH 7). Enzyme activity was determined using 2.5 mM 2,2\u2019-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 1 mM H2O2 and 50 mM citrate. Samples were shaken during turnover to ensure sufficient substrate supply. Every two minutes in a total of ten minutes reaction time, aliquots of 100 \u03bcl were removed and measured at 405 nm using a microplate reader (Biotek Epoch). Enzyme activity was calculated based on the linear slope of the kinetic. Means and standard deviations reflect three experiments.\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Thu, 02\/06\/2025 - 10:16","name":"Supplementary Figure5","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"30.03 KB","created":"Tue, 02\/04\/2025 - 14:08","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Thu, 02\/06\/2025 - 10:16"}],"groups":[{"description":"","id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","image_display_url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/rublogoweiss_0_1.png","title":"Applied Microbiology","name":"group\/applied-microbiology"}]}]}