{"help":"Return the metadata of a dataset (package) and its resources. :param id: the id or name of the dataset :type id: string","success":true,"result":[{"id":"18687f98-d046-4c8b-8e12-3f45d4b2d128","name":"plasma\u2010driven-\u2005situ-production-hydrogen-peroxide-biocatalysis","title":"Plasma\u2010driven in\u2005situ production of hydrogen peroxide for biocatalysis","author_email":"julia.bandow@rub.de","maintainer":"Research Data Repository","maintainer_email":"achim.vonkeudell@rub.de","license_title":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/","notes":"\u003Cp\u003EPeroxidases and peroxygenases are promising classes of enzymes for biocatalysis because of their ability to carry out one\u2010electron oxidation reactions and stereoselective oxyfunctionalizations. However, industrial application is limited, as the major drawback is the sensitivity toward the required peroxide substrates. Herein, we report a novel biocatalysis approach to circumvent this shortcoming: in\u2005situ production of H2O2 by dielectric barrier discharge plasma. The discharge plasma can be controlled to produce hydrogen peroxide at desired rates, yielding desired concentrations. Using horseradish peroxidase, it is demonstrated that hydrogen peroxide produced by plasma treatment can drive the enzymatic oxidation of model substrates. Fungal peroxygenase is then employed to convert ethylbenzene to (R)\u20101\u2010phenylethanol with an ee of \u0026gt;96\u2009% using plasma\u2010generated hydrogen peroxide. As direct treatment of the reaction solution with plasma results in reduced enzyme activity, the use of plasma\u2010treated liquid and protection strategies are investigated to increase total turnover. Technical plasmas present a noninvasive means to drive peroxide\u2010based biotransformations.\u003C\/p\u003E\n","url":"https:\/\/rdpcidat.rub.de\/dataset\/plasma%E2%80%90driven-%E2%80%85situ-production-hydrogen-peroxide-biocatalysis","state":"Active","log_message":"Update to resource Figure 4. CD spectra of HRP after exposure to plasma","private":true,"revision_timestamp":"Sun, 03\/21\/2021 - 19:21","metadata_created":"Wed, 11\/18\/2020 - 11:06","metadata_modified":"Sun, 03\/21\/2021 - 19:21","creator_user_id":"2282a879-bb83-4efc-a40f-ecca445b4ed0","type":"Dataset","resources":[{"id":"4ee5ca6d-f231-462b-b0f8-b1fbd478c292","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/HRP%20direct%20plasma%2020170529%20paper%20%2B%20H2O2.xlsx","description":"\u003Cp\u003ESamples were placed onto glass slides and treated for the indicated amount\u003Cbr \/\u003E\nof time. Black: 50 mm KPi buffer (100 mL) was treated with plasma. Immediately\u003Cbr \/\u003E\nafter treatment, the samples (20 mL) were mixed with 180 mL A. dest.\u003Cbr \/\u003E\nand H2O2 concentrations determined using the Spectroquant Hydrogen Peroxide\u003Cbr \/\u003E\nkit (Merck) and photometrical measurements at 455 nm. Red: direct\u003Cbr \/\u003E\nconversion of guaiacol (5 mm) with plasma was achieved by treating KPi\u003Cbr \/\u003E\nbuffer (100 mL) containing 0.1 UmL@1 HRP. Production of tetraguaiacol was\u003Cbr \/\u003E\nfollowed at l=470 nm. The data shown represent means of three independent\u003Cbr \/\u003E\nexperiments.\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Sun, 03\/21\/2021 - 14:51","name":"Fig. 2 Kinetics of H2O2 accumulation and substrate conversion by HRP during direct plasma treatment.","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"17.75 KB","created":"Wed, 11\/18\/2020 - 12:28","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Sun, 03\/21\/2021 - 14:51"},{"id":"7e433971-9ace-465c-bc3c-390d7720b16f","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/HRP%20inactivation%20and%20absorption%20spectrum%20plasma%2020180214.xlsx","description":"\u003Cp\u003EPlasma treatment was performed\u003Cbr \/\u003E\nwith 110 mL HRP (1 kUmL@1) in KPi buffer (100 mm, pH 6.5) without\u003Cbr \/\u003E\nguaiacol. Activity was measured by diluting the treated samples to\u003Cbr \/\u003E\n0.1 UmL@1 in KPi buffer including 5 mm of guaiacol and subsequent addition\u003Cbr \/\u003E\nof H2O2 at a final concentration of 0.25 mm. Guaiacol conversion was immediately\u003Cbr \/\u003E\nmonitored at l=470 nm and activity calculated from the initial slope\u003Cbr \/\u003E\nduring the first 30 s. From the same treated samples, absorption spectra\u003Cbr \/\u003E\nwere measured with a 1:5 dilution in KPi. The inset shows the Soret band of\u003Cbr \/\u003E\nHRP with Amax at \u0026amp;403 nm. Spectra are displayed with the untreated sample\u003Cbr \/\u003E\nas blank, thereby showing a decrease in absorbance at the Soret peak. Data\u003Cbr \/\u003E\nwere recorded in triplicate for both activity measurements and spectra.\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Sun, 03\/21\/2021 - 19:22","name":"Figure 3. HRP inactivation by plasma treatment","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"427.93 KB","created":"Wed, 11\/18\/2020 - 12:33","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Sun, 03\/21\/2021 - 19:22"},{"id":"4ab012f3-735b-4aad-9a60-a8f9b8bcd380","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/CD%20spec%20HRP%2020180118.xlsx","description":"\u003Cp\u003EHRP (110 mL) was\u003Cbr \/\u003E\ntreated as described above for activity measurements and diluted 1:5, which\u003Cbr \/\u003E\ncorresponds to 0.2 mgmL@1 for the untreated sample. Immediately after\u003Cbr \/\u003E\ntreatment, the sample was transferred to a suitable cuvette and subjected\u003Cbr \/\u003E\nto CD measurements. CD spectra were normalized with respect to protein\u003Cbr \/\u003E\nconcentration as determined by the Bradford method.\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Sun, 03\/21\/2021 - 19:21","name":"Figure 4. CD spectra of HRP after exposure to plasma","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"1.4 MB","created":"Wed, 11\/18\/2020 - 12:35","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Sun, 03\/21\/2021 - 19:21"},{"id":"2102d010-9e77-427c-97cf-4fe3a8db921c","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/HRP%20bradford%20and%20specific%20heme%20content%20plasma%2020180214.xlsx","description":"\u003Cp\u003EProtein concentration\u003Cbr \/\u003E\nwas determined using the Bradford method. Specific heme content\u003Cbr \/\u003E\nwas set as absorbance at l=403 (see Figure 3) divided by protein concentration\u003Cbr \/\u003E\nand is displayed in arbitrary units. Protein concentration was determined\u003Cbr \/\u003E\nin triplicate.\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Sun, 03\/21\/2021 - 19:20","name":"Figure 5. Decrease in protein concentration and heme content.","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"36.12 KB","created":"Wed, 11\/18\/2020 - 12:36","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Sun, 03\/21\/2021 - 19:20"},{"id":"92534137-659b-4d89-adb3-9c9fdaf41fe4","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/HRP%20Plasmabehandlung%20Aktivit%C3%A4t%20verschiedene%20Parameter%20Zusammenfassung.xlsx","description":"\u003Cp\u003EHRP was plasma-treated at 10 UmL@1 for 1 min and activity was\u003Cbr \/\u003E\nsubsequently measured ex situ as described above. Untreated samples were\u003Cbr \/\u003E\nset to 100%. The graph shows mean values of three independent replicates.\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Sun, 03\/21\/2021 - 19:20","name":"Figure 6. Influence of voltage and frequency on HRP after 1 min plasma treatment","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"61.62 KB","created":"Wed, 11\/18\/2020 - 12:37","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Sun, 03\/21\/2021 - 19:20"},{"id":"760f92cc-576b-4d50-8454-9bd3f8e54bd1","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/HRP%20immobilisierung%20plasma%2020180601.xlsx","description":"\u003Cp\u003EGlutaraldehyde-activated polymethacrylate beads (Relizyme HA403) were incubated\u003Cbr \/\u003E\nwith HRP overnight to yield an immobilized HRP solution corresponding\u003Cbr \/\u003E\nto 20 UmL@1. For both enzyme formulations, 100 mL was treated\u003Cbr \/\u003E\nat 13.5 kV and 300 Hz. Free enzyme activity was measured as described\u003Cbr \/\u003E\nabove. Activity assays for immobilized HRP were conducted with constant\u003Cbr \/\u003E\nshaking to provide sufficient substrate delivery to the macroscopic beads,\u003Cbr \/\u003E\nusing 5 mm guaiacol and 0.25 mm H2O2. The displayed relative activities\u003Cbr \/\u003E\nwere calculated by relating the activities of treated samples to their respective\u003Cbr \/\u003E\nuntreated controls. Data represent means of three replicates.\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Sun, 03\/21\/2021 - 19:19","name":"Figure 7. Immobilization of HRP protects against plasma-mediated damage","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"29.5 KB","created":"Wed, 11\/18\/2020 - 12:40","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Sun, 03\/21\/2021 - 19:19"},{"id":"7bda10d3-75d6-4420-b7ad-bbf616d24ebc","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/UPO%20immobilized%20supernatant%20cycles.xlsx","description":"\u003Cp\u003ESupernatant\u003Cbr \/\u003E\nof the reaction vial, that is, buffer without enzyme, was treated as mentioned\u003Cbr \/\u003E\nbefore and added back into the container. This was repeated for\u003Cbr \/\u003E\nseveral cycles as indicated. Turnover of ethylbenzene to (R)-1-phenylethanol\u003Cbr \/\u003E\nwas allowed to take place for 30 min after a new cycle was initiated. (R)-1-\u003Cbr \/\u003E\nPhOl: (R)-1-phenylethanol.\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Sun, 03\/21\/2021 - 19:19","name":"Figure 8. Production of (R)-1-phenylethanol with immobilized rAaeUPO using plasma-treated KPi buffer (250 mm) treated for several cycles","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"15.27 KB","created":"Wed, 11\/18\/2020 - 12:41","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Sun, 03\/21\/2021 - 19:19"},{"id":"5c157c35-81d7-42bc-9651-c540f9b24df1","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/HRP%20direct%20plasma%2020170529%20paper.xlsx","description":"\u003Cp\u003EReactions of 100 \u03bcl were run without enzyme, without plasma treatment, or with both enzyme\u003Cbr \/\u003E\nand plasma treatment for 5 min (composition: 0.1 U ml-1 HRP, 5 mM guaiacol, 50 mM KPi pH\u003Cbr \/\u003E\n6.5). At the indicated time points, samples were withdrawn and absorption was measured at\u003Cbr \/\u003E\n470 nm. n=3 for the samples with plasma and HRP, n=1 for either HRP or plasma only.\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Sun, 03\/21\/2021 - 19:18","name":"Supplementary Figure 1. Background activity of direct biocatalysis using HRP.","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"15.8 KB","created":"Wed, 11\/18\/2020 - 12:44","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Sun, 03\/21\/2021 - 19:18"},{"id":"4a833768-71cb-4fe3-bea8-a85994342f92","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/HRP%20Heme%20extraction%2020180723.xlsx","description":"\u003Cp\u003EIn\u003Cbr \/\u003E\norder to extract the heme from HRP, acidified enzyme solution was incubated with an excess\u003Cbr \/\u003E\nof ethyl acetate for 1 min. After centrifugation, the aqueous phase was transferred and protein\u003Cbr \/\u003E\ncontent was determined by the Bradford method. Catalysis was then carried out using\u003Cbr \/\u003E\nuntreated (control) and heme-depleted HRP in 5 mM of guaiacol in 50 mM KPi buffer. After 5\u003Cbr \/\u003E\nmin of plasma treatment, absorption at 470 nm was determined and normalized to protein\u003Cbr \/\u003E\ncontent of the sample as well as treatment time. The experiment was performed 3 times\u003Cbr \/\u003E\nindependently.\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Sun, 03\/21\/2021 - 19:18","name":"Supplementary Figure 2. Comparison of active and inactive HRP in direct catalysis.","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"44.17 KB","created":"Wed, 11\/18\/2020 - 12:50","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Sun, 03\/21\/2021 - 19:18"},{"id":"535eaacd-244f-4ef2-9d2c-dd5cf35746cc","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/200127%20Spektrum%20HRP%20direct%20catalysis.xlsx","description":"\u003Cp\u003EA mixture\u003Cbr \/\u003E\nof 0.1 U ml-1 HRP and 5 mM of guaiacol in 50 mM KPi buffer was either treated with the DBD\u003Cbr \/\u003E\nplasma source for 5 min (black), incubated with 0.5 mM H2O2 (red), or left untreated (green).\u003Cbr \/\u003E\nThe spectra were recorded immediately after completion of the reaction. The experiment was\u003Cbr \/\u003E\nperformed three times. Representative spectra are shown.\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Sun, 03\/21\/2021 - 19:18","name":"Supplementary Figure 3. Spectrum of reaction products of HRP and guaiacol.","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"90.32 KB","created":"Wed, 11\/18\/2020 - 12:52","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Sun, 03\/21\/2021 - 19:18"},{"id":"d262cbb8-9ffe-43b8-a73a-3bd4ad230103","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/HRP%20substrates%20test%2020170321.xlsx","description":"\u003Cp\u003E100 \u03bcl of 100 mM pyrogallol, 10 mM guaiacol, and 20 mM L-DOPA in 100 mM KPi buffer were\u003Cbr \/\u003E\ntreated with plasma for the indicated amounts of time. After treatment, absorption was\u003Cbr \/\u003E\nmeasured immediately at 420 nm, 470 nm, and 450 nm, respectively. n=1.\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Sun, 03\/21\/2021 - 19:17","name":"Supplementary Figure 4. Conversion of substrates by plasma in the absence of enzyme.","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"67 KB","created":"Wed, 11\/18\/2020 - 12:53","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Sun, 03\/21\/2021 - 19:17"},{"id":"7c37bd58-fb4a-497c-ae3d-2403d38f7b42","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/HRP%20Plasmabehandlung%20Aktivit%C3%A4t%20nach%20Proteinkonzentration%20201808.xlsx","description":"\u003Cp\u003EHRP was diluted to different concentrations and treated with plasma for the\u003Cbr \/\u003E\nindicated amounts of time (100 \u03bcl). Activity of HRP was determined subsequently using a final\u003Cbr \/\u003E\nconcentration of 0.5 mM H2O2 and 5 mM guaiacol. Activity of untreated samples was set to\u003Cbr \/\u003E\n100%. The figure shows mean values of three independent experiments.\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Sun, 03\/21\/2021 - 19:16","name":"Supplementary Figure 5. HRP inactivation kinetics at different protein concentrations during treatment.","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"82.01 KB","created":"Wed, 11\/18\/2020 - 12:54","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Sun, 03\/21\/2021 - 19:16"},{"id":"58064e4c-d260-453f-ad2f-2e1329fd8ee3","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/UPO%20SodA%20paper.xlsx","description":"\u003Cp\u003ESodA was added to the\u003Cbr \/\u003E\nstandard reaction mixture (10 U ml-1, 5 mM guaiacol, 100 mM KPi) at 1 mg ml-1 prior to plasma\u003Cbr \/\u003E\ntreatment. After 5 min of plasma treatment, A470 was measured to quantify product formation.\u003Cbr \/\u003E\nThe experiment was performed three times.\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Sun, 03\/21\/2021 - 19:16","name":"Supplementary Figure 6. Influence of SodA on HRP activity.","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"90.1 KB","created":"Wed, 11\/18\/2020 - 12:56","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Sun, 03\/21\/2021 - 19:16"},{"id":"7a7c8305-abca-4051-b2a5-2d9311842f4c","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/Mannitol%20-%20HRP%20Plasmabehandlung%20Aktivit%C3%A4t.xlsx","description":"\u003Cp\u003EPlasma treatment was performed with 100 \u03bcl of 10 U ml-1 HRP with or\u003Cbr \/\u003E\nwithout the addition of 100 mM mannitol as \u2022OH scavenger. Activities were determined as\u003Cbr \/\u003E\ndescribed above (Fig. S5). Values shown resemble mean values of three replicates.\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Sun, 03\/21\/2021 - 19:16","name":"Supplementary Figure 7. Relative activity of HRP after plasma treatment with and without mannitol.","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"83.05 KB","created":"Wed, 11\/18\/2020 - 12:56","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Sun, 03\/21\/2021 - 19:16"},{"id":"189e17a7-60ad-4815-8e49-f7caefb85de1","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/HRP%20immobilisierung%20plasma%2020180601_0.xlsx","description":"\u003Cp\u003EImmobilization\u003Cbr \/\u003E\nwas carried out as described in the Methods section. Activity assays were performed as\u003Cbr \/\u003E\ndescribed above, using 5 mM guaiacol, 0.5 mM H2O2 as well as 0.1 and 1 U ml-1 HRP,\u003Cbr \/\u003E\nrespectively. For the immobilized formulation, activity assays were performed under agitation\u003Cbr \/\u003E\nto ensure sufficient substrate flux to the beads. Activity was normalized to enzyme\u003Cbr \/\u003E\nconcentration.\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Sun, 03\/21\/2021 - 19:12","name":"Supplementary Figure 8. Relative activity of free and immobilized HRP.","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"35.81 KB","created":"Wed, 11\/18\/2020 - 13:00","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Sun, 03\/21\/2021 - 19:12"},{"id":"4150d55c-d826-4e55-afd7-ab65bdd4bae4","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/GC%20Data%20Summary.xlsx","description":"\u003Cp\u003EA reaction mixture of 300 \u03bcl plasma-treated buffer, 5 \u03bcl ethylbenzene, and 50 \u03bcl of 1 \u03bcM rAaeUPO were mixed and incubated for a total time of 30 min. The final reaction volume was extracted with 300 \u03bcl ethyl acetate containing 2 mM of 1-octanol as injection standard. The organic phase was transferred to a new vial, dried with MgSO4 and measured with a Shimadzu 2010 GC system containing a Hydrodex \u03b2-6TBDM column (Macherey-Nagel, Germany). Using plasma-treated buffer and rAaeUPO, only the R-enantiomer of 1-phenylethanol was detected (light blue). Omitting either plasma treatment (red), substrate (green) or rAaeUPO (purple) did not yield any product (R-PhOl: (R)-1-phenylethanol).\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Sun, 03\/21\/2021 - 19:15","name":"Supplementary Figure 9. Analysis of reaction products of rAaeUPO and plasma-treated buffer.","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"5.45 MB","created":"Wed, 11\/18\/2020 - 13:02","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Sun, 03\/21\/2021 - 19:15"},{"id":"2b7776f7-2b45-4de9-84cc-d8818b610103","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/200128%20UPO%20incubation%20in%20H2O2_PTB.xlsx","description":"\u003Cp\u003E110 \u03bcl of buffer were treated for 10 min as described before and H2O2\u003Cbr \/\u003E\nconcentration was measured using the Spectroquant kit. Then, rAaeUPO was added at 40 nM\u003Cbr \/\u003E\nand incubated for 2 min. For the sample using diluted H2O2, the same concentration of H2O2\u003Cbr \/\u003E\nwas used as had been measured in the plasma-treated samples. ABTS was added as\u003Cbr \/\u003E\nsubstrate and activity was measured as described before, substituting sodium acetate buffer\u003Cbr \/\u003E\nfor potassium phosphate buffer (pH 7) and using only the H2O2 already present in the samples.\u003Cbr \/\u003E\nActivity of rAaeUPO without incubation represents 100%. n=3.\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Sun, 03\/21\/2021 - 19:14","name":"Supplementary Figure 10. Relative activity of rAaeUPO after incubation in plasmatreated buffer.","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"30.71 KB","created":"Wed, 11\/18\/2020 - 13:03","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Sun, 03\/21\/2021 - 19:14"},{"id":"b1374772-5313-4f10-a697-41a1dc48d1d5","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/UPO%20HRP%20plasma%20treatment%20inactivation.xlsx","description":"\u003Cp\u003EPlasma\u003Cbr \/\u003E\nexposure was performed as described for HRP. Enzyme solution was then diluted 1:10 in 100\u003Cbr \/\u003E\nmM sodium acetate buffer containing 2.5 mM ABTS as substrate. Absorption was followed at\u003Cbr \/\u003E\n405 nm immediately after adding H2O2 at a final concentration of 1 mM. Background activity\u003Cbr \/\u003E\nwas determined by adding deionized water instead of H2O2. Background activity was\u003Cbr \/\u003E\nsubtracted in all measurements. Data for HRP is taken from Fig 3. 100% represents activity of\u003Cbr \/\u003E\nuntreated samples. Three independent replicates were performed.\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Sun, 03\/21\/2021 - 19:14","name":"Supplementary Figure 11. Relative activity of rAaeUPO after plasma treatment.","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"59.67 KB","created":"Wed, 11\/18\/2020 - 13:04","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Sun, 03\/21\/2021 - 19:14"},{"id":"03e18ba1-015e-47be-8a85-ed0a675e0447","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/UPO%20SodA%20paper.xlsx","description":"\u003Cp\u003EPlasma treatment of rAaeUPO was performed either without\u003Cbr \/\u003E\nor in the presence of 1 mg ml-1 SodA for 5 min using 100 \u03bcl. Subsequently, samples were\u003Cbr \/\u003E\ndiluted 1:10 in sodium acetate buffer (pH 5.5) containing 2.5 mM ABTS. After addition of H2O2\u003Cbr \/\u003E\nat a final concentration of 1 mM, A405 was followed for 5 min. Activities were calculated from\u003Cbr \/\u003E\nthe initial slope. The residual activity was defined as the activity of the treated samples divided\u003Cbr \/\u003E\nby the activity of the respective untreated samples. n=2.\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Sun, 03\/21\/2021 - 19:14","name":"Supplementary Figure 12. Relative activity of rAaeUPO after plasma treatment in the presence of SodA from E. coli.","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"90.1 KB","created":"Wed, 11\/18\/2020 - 13:05","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Sun, 03\/21\/2021 - 19:14"},{"id":"63423177-4aa5-435c-9054-95d4b813d01b","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/UPO%20immobilisierung%20plasma.xlsx","description":"\u003Cp\u003EImmobilized rAaeUPO\u003Cbr \/\u003E\nwas diluted to 1 \u03bcM and 100 \u03bcl were treated with plasma for the indicated amounts of time.\u003Cbr \/\u003E\nBeads were washed off the glass support and used for activity measurements using 2.5 mM\u003Cbr \/\u003E\nABTS and 1 mM H2O2 in 50 mM sodium acetate buffer. Activity of untreated immobilized\u003Cbr \/\u003E\nrAaeUPO was set to 100%. Data shown represent mean values of three independent\u003Cbr \/\u003E\nreplicates.\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Sun, 03\/21\/2021 - 19:13","name":"Supplementary Figure 13. Inactivation of immobilized rAaeUPO.","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"38.67 KB","created":"Wed, 11\/18\/2020 - 13:06","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Sun, 03\/21\/2021 - 19:13"},{"id":"6a0e96d1-22da-4bea-aa25-91a872f5d85d","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/UPO%20immobilisierung%20Aktivit%C3%A4tstest.xlsx","description":"\u003Cp\u003EActivities of free and immobilized rAaeUPO were measured using 2.5 mM ABTS, 1 mM H2O2\u003Cbr \/\u003E\nin 50 mM sodium acetate buffer as well as 1 or 10 nM enzyme, respectively. Activity was\u003Cbr \/\u003E\nmeasured from the same stock of immobilized rAaeUPO in three independent replicates.\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Sun, 03\/21\/2021 - 19:13","name":"Supplementary Figure 14. Relative activity of rAaeUPO before and after immobilization.","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"65.51 KB","created":"Wed, 11\/18\/2020 - 13:07","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Sun, 03\/21\/2021 - 19:13"},{"id":"63978a3d-166e-409f-b8a1-8f892dfdb4b7","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/190115%20calibration%20and%20UPO.xlsx","description":"\u003Cp\u003EEither 110 \u03bcl of plasma-exposed (5 min treatment time) KPi buffer\u003Cbr \/\u003E\n(blue) or 0.7 mM H2O2 in KPi (red) were transferred to a vial containing 5 \u03bcl ethylbenzene and\u003Cbr \/\u003E\nimmobilized rAaeUPO. The samples were incubated for 15 min with constant shaking before\u003Cbr \/\u003E\ntransferring the supernatant to another vial. The supernatant was extracted with the same\u003Cbr \/\u003E\nvolume of ethyl acetate containing 2 mM 1-octanol as internal standard and dried with MgSO4.\u003Cbr \/\u003E\nThe same sample of immobilized rAaeUPO was then used for another cycle of conversion in\u003Cbr \/\u003E\na new reaction solution. The experiment was performed three times independently.\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Sun, 03\/21\/2021 - 19:12","name":"Supplementary Figure 15. Conversion of ethylbenzene to (R)-1-phenylethanol with immobilized rAaeUPO.","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"81.79 KB","created":"Wed, 11\/18\/2020 - 13:08","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Sun, 03\/21\/2021 - 19:12"}],"tags":[{"id":"55db12b1-2415-4c44-a3c5-89161362841f","vocabulary_id":"2","name":"biocatalysis"},{"id":"56149987-dd82-4887-aea1-955278b5425c","vocabulary_id":"2","name":"peroxidase"},{"id":"7a08ead4-cd95-411a-a555-944c523951d8","vocabulary_id":"2","name":"peroxygenase"},{"id":"b1bfc206-d178-4e3a-9dcc-8819a1bf54be","vocabulary_id":"2","name":"plasma chemistry"},{"id":"f35c8e50-f7c8-4def-a567-31b0068faacb","vocabulary_id":"2","name":"peroxide"}],"groups":[{"description":"","id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","image_display_url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/rublogoweiss_0_1.png","title":"Applied Microbiology","name":"group\/applied-microbiology"}]}]}