{"help":"Return the metadata of a dataset (package) and its resources. :param id: the id or name of the dataset :type id: string","success":true,"result":[{"id":"0f0329d3-7134-4a7b-9c56-ad1a36bb5705","name":"nanosecond-pulsed-microwave-plasma-torch-situ-h2o2-supply-path-stable-and-scalable-plasma","title":"Nanosecond pulsed microwave plasma torch for in situ H2O2 supply: A path to stable and scalable plasma-driven biocatalysis","author_email":"tim.dirks@rub.de","maintainer":"Research Data Repository","maintainer_email":"achim.vonkeudell@rub.de","license_title":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/","notes":"\u003Cp\u003EPlasma-driven in situ H2O2 generation offers a potential sustainable route for oxidative biotransformations, but its application has been hindered by challenges such as insufficient H2O2 production and enzyme degradation from direct plasma exposure. This study establishes plasma-driven biocatalysis using a nanosecond pulsed microwave plasma torch (npMWPT) as a plasma source, highlighting its unique advantages over previously applied sources. npMWPT-driven biocatalysis addresses insufficient plasma-generated H2O2 production by enabling a continuous and ample H2O2 supply while decoupling plasma treatment from the enzymatic reaction. This setup significantly extends enzyme stability, thus supporting prolonged biocatalytic activity. We investigated the effectiveness of npMWPT-driven biocatalysis using both immobilized and free recombinant unspecific peroxygenase from Agrocybe aegerita (rAaeUPO) to convert ABTS and ethylbenzene. For ABTS, immobilized rAaeUPO demonstrated consistent reusability across five reaction cycles (TON 44,637 \u00b5molABTS* \u00b5mol-1rAaeUPO). The decoupling of npMWPT-based H2O2 production resulted in a turnover number of 66,495 \u00b5mol product per \u00b5mol enzyme with ethylbenzene for free rAaeUPO, outperforming the use of immobilized rAaeUPO for the first time in plasma-driven biocatalysis (TTN of 22,116 \u00b5mol(R)1-PhOl \u00b5mol-1rAaeUPO). Mixing optimization further improved ethylbenzene conversion rates, although the continuous H2O2 flow eventually led to enzyme inactivation due to excess H2O2. These findings highlight the dual advantages of the npMWPT-driven biocatalysis in enhancing enzyme longevity and ensuring sustained H2O2 supply, creating a more favorable environment for biocatalysis. This proof-of-principle study demonstrates the successful integration of the npMWPT plasma source in plasma-driven biocatalysis, establishing a viable setup that provides a promising pathway for scaling up plasma-driven biocatalysis for complex biotransformations.\u003C\/p\u003E\n","url":"https:\/\/rdpcidat.rub.de\/dataset\/nanosecond-pulsed-microwave-plasma-torch-situ-h2o2-supply-path-stable-and-scalable-plasma","state":"Active","log_message":"Edited by kd.","private":true,"revision_timestamp":"Thu, 10\/30\/2025 - 14:24","metadata_created":"Thu, 10\/23\/2025 - 12:03","metadata_modified":"Thu, 10\/30\/2025 - 14:24","creator_user_id":"fbaa0bb1-6827-46c5-a646-bca2c4ce442a","type":"Dataset","resources":[{"id":"8ae2e76f-6de1-435a-abc5-6f235053ca03","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/Figure_1.xlsx","description":"\u003Cp\u003ESubstrate (ABTS) conversion performing plasma-driven biocatalysis using immobilized rAaeUPO with npMWPT. (a) Conversion of ABTS using immobilized rAaeUPO was tested by transferring 100 mg protein-loaded beads to a rotating bed reactor, which was placed in a vessel filled with 1 ml ABTS (5 mmol l-1) dissolved in 100 mmol l-1 citrate buffer (pH 5). Plasma-treated water was added at a flow rate of 2.5 ml min 1 for 2 min, while the absorbance was measured every 30 s at 405 nm. To prove the reusability of the rAaeUPO-loaded beads, the whole reaction solution was withdrawn from the vessel and the reaction cycle was repeated (total cycle amount of five). The total product amount was calculated under consideration of the introduced dilution factor and based on the millimolar extinction coefficient (\u03b5 = 36.8\u2009mmol l\u22121 cm\u22121). (b) Accumulation of formed product (ABTS* in \u00b5mol) over the total run time of 600 s in five reaction cycles. Means and standard deviations reflect three independent experiments. Standard deviations that are not visible were below 0.02 \u00b5mol (a) or 0.1 \u00b5mol (b).\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Thu, 10\/30\/2025 - 11:08","name":"Figure 1: Substrate (ABTS) conversion performing plasma-driven biocatalysis using immobilized rAaeUPO with npMWPT.","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"49.21 KB","created":"Thu, 10\/23\/2025 - 12:06","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Thu, 10\/30\/2025 - 11:08"},{"id":"1ca3ffb3-c47e-4e46-a2c8-1ec2ed332cb7","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/Figure_2.xlsx","description":"\u003Cp\u003EPlasma-treated water (1.5 ml) was mixed with 50 mmol l-1 ethylbenzene and 50 or 100 nmol l-1 (0.075 or 0.15 nmol) free rAaeUPO. After 10 min incubation under rapid mixing, 150 \u00b5l sample was withdrawn for gas chromatography (GC) analysis. Means and standard deviations represent three independent experiments.\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Thu, 10\/30\/2025 - 11:07","name":"Figure 2 Ethylbenzene conversion using npMWPT-treated water in combination with free rAaeUPO","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"22.14 KB","created":"Thu, 10\/23\/2025 - 12:06","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Thu, 10\/30\/2025 - 11:07"},{"id":"1aa5efb8-f60b-4a4e-ad65-545e8949996d","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/Figure_3.xlsx","description":"\u003Cp\u003EEthylbenzene (50 mmol l-1) was mixed with free rAaeUPO (0.1 nmol) in a total volume of 1 ml potassium phosphate buffer (100 mmol l-1, pH 7). Continuous flow of npMWPT-treated water at a flow rate of 2.5 ml min-1 was added over a time frame of 2 min, while the solution was mixed using an empty rotating bed reactor at 900 rpm. After 2 min, the flow of plasma-treated water was stopped. Samples (150 \u00b5l) were withdrawn for GC analysis directly after 2 min (\u2018after reaction\u2019) and after further 10 min incubation (\u2018after incubation\u2019) to allow conversion of residual H2O2. Means and standard deviations reflect three independent experiments.\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Thu, 10\/30\/2025 - 11:07","name":"Figure 3: Ethylbenzene conversion using continuous flow of npMWPT-treated water in combination with free rAaeUPO. ","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"24.87 KB","created":"Thu, 10\/23\/2025 - 12:07","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Thu, 10\/30\/2025 - 11:07"},{"id":"24628d92-0491-44a6-b97e-5cb00f2f3615","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/Figure_4.xlsx","description":"\u003Cp\u003EConversion of ethylbenzene with immobilized rAaeUPO was analyzed by loading 200 mg enzyme-loaded beads into a rotating bed reactor. The flow of substrate (100 mmol l-1 ethylbenzene in 100 mmol l-1 potassium phosphate buffer, pH 7) was combined with the continuous flow of the plasma-treated water (total flow rate of 5 ml min 1) and continuously supplied to the reaction vessel with the rotating bed reactor for a total run time of 30 min. Every 45 s, samples of 3.75 ml were withdrawn to prevent overflowing of the reaction vessel, while four samples were pooled in one reaction tube (total volume of 15 ml). (a) To analyze product formation in the respective 3 min time frame, 150 \u00b5l sample was withdrawn for GC measurement. (b) Accumulation of (R)-1-PhOl performing plasma-driven biocatalysis calculated by addition of all measured (R)-1-PhOl amounts during the 30 min biocatalysis. Means and standard deviations represent three independent experiments.\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Thu, 10\/30\/2025 - 11:08","name":"Figure 4: Substrate (ethylbenzene) conversion performing plasma-driven biocatalysis using immobilized rAaeUPO with npMWPT","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"50.91 KB","created":"Thu, 10\/23\/2025 - 12:07","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Thu, 10\/30\/2025 - 11:08"},{"id":"6bcbc592-a75a-4582-83e0-9df13bef100f","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/Suppl_Figure_4.xlsx","description":"\u003Cp\u003EEthylbenzene (50 mmol l-1) was mixed with free rAaeUPO (0.1 nmol) in a total volume of 1 ml potassium phosphate buffer (100 mmol l-1, pH 7). Continuous flow of npMWPT-treated water at a flow rate of 2.5 ml min-1 was added over a time frame of 2 min, while the solution was mixed using a magnetic stirrer at 900 rpm. After 2 min, the flow of plasma-treated water was stopped. Samples (150 \u00b5l) were withdrawn for GC analysis directly after 2 min (\u2018after reaction\u2019) and after further 10 min incubation (\u2018after incubation\u2019) to allow conversion of residual H2O2. Means and standard deviations reflect three independent experiments.\u003C\/p\u003E\n","format":"xlsx","state":"Active","revision_timestamp":"Thu, 10\/30\/2025 - 11:08","name":"Supplementary Figure 4: Ethylbenzene conversion using continuous flow of npMWPT-treated water in combination with free rAaeUPO","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"21.67 KB","created":"Thu, 10\/23\/2025 - 12:08","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Thu, 10\/30\/2025 - 11:08"},{"id":"ed113cd1-7aa2-43ad-9f4e-de1795f2c59d","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/Table_1.xlsx","description":"","format":"xlsx","state":"Active","revision_timestamp":"Thu, 10\/30\/2025 - 11:08","name":"Table 1: Product formation and kinetic parameters of rAaeUPO catalyzed ABTS conversion using npMWPT-treated water as H2O2 source.","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"16.57 KB","created":"Thu, 10\/23\/2025 - 12:08","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Thu, 10\/30\/2025 - 11:08"},{"id":"657a4710-b02e-40b9-88c1-48802626c7f0","revision_id":"","url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/Table_2.xlsx","description":"","format":"xlsx","state":"Active","revision_timestamp":"Thu, 10\/30\/2025 - 11:08","name":"Table 2: Product formation and kinetic parameters of rAaeUPO (0.15 nmol) catalyzed ethylbenzene conversion using npMWPT-treated water as H2O2 source.","mimetype":"application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet","size":"22.24 KB","created":"Thu, 10\/23\/2025 - 12:08","resource_group_id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","last_modified":"Date changed  Thu, 10\/30\/2025 - 11:08"}],"tags":[{"id":"7744ce95-8a41-43da-aa7b-cbfb325be3b3","vocabulary_id":"2","name":"Plasma-driven biocatalysis"}],"groups":[{"description":"","id":"a7cc37b6-5294-4469-8ad1-7e60df6ea28f","image_display_url":"https:\/\/rdpcidat.rub.de\/sites\/default\/files\/rublogoweiss_0_1.png","title":"Applied Microbiology","name":"group\/applied-microbiology"}]}]}